| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CT13; HAGE |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DDX43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与DDX43抗体相关的代表性文献(模拟摘要内容，实际引用请核对原文)：
1. **文献名称**：DDX43 as a tumor-associated antigen in melanoma and its antibody application in diagnosis
**作者**：Smith A et al.
**摘要**：研究验证了DDX43蛋白在黑色素瘤组织中的特异性高表达，开发的小鼠单克隆抗体在免疫组化中表现出高灵敏度和特异性，可作为辅助诊断标志物。
2. **文献名称**：Characterization of a novel anti-DDX43 antibody for detecting cancer stem cells in colorectal cancer
**作者**：Chen L et al.
**摘要**：报道了一种新型兔源多克隆抗体的制备，通过Western blot和流式细胞术证实该抗体可特异性识别结直肠癌干细胞中的DDX43蛋白，提示其在肿瘤干细胞研究中的应用潜力。
3. **文献名称**：DDX43 antibody-based liquid biopsy for early detection of ovarian cancer
**作者**：Wang Y et al.
**摘要**：利用自主研发的DDX43单克隆抗体建立ELISA检测方法，在卵巢癌患者血清中检测到异常升高的DDX43蛋白水平，灵敏度达82%，为无创早筛提供新策略。
注：以上为模拟文献摘要，实际文献需通过PubMed/Google Scholar检索关键词"DDX43 antibody" "cancer"等获取。部分真实研究可参考：PMID 29477803、PMID 26804793等(需核实具体内容)。
The DDX43 antibody targets the DEAD-box helicase 43 (DDX43), also known as HAGE (helicase antigen), a protein encoded by the DDX43 gene. DDX43 belongs to the DEAD-box RNA helicase family, which is involved in RNA metabolism, including unwinding RNA secondary structures, splicing, and translation. Unlike many DEAD-box proteins, DDX43 is classified as a cancer-testis antigen due to its restricted expression in normal tissues (primarily testis) and frequent upregulation in various cancers, such as melanoma, leukemia, and solid tumors. Its overexpression in malignancies has linked it to tumorigenesis, proliferation, and metastasis, making it a potential diagnostic or prognostic biomarker.
DDX43 antibodies are essential tools for studying its expression patterns, localization, and functional roles in cancer biology. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect DDX43 in clinical samples or experimental models. Additionally, these antibodies support research into DDX43's interaction with RNA/DNA and its involvement in epigenetic regulation. Given its immunogenic properties, DDX43 is also explored as a target for cancer immunotherapy, including vaccine development. However, antibody specificity remains a critical consideration, as cross-reactivity with other DEAD-box proteins may occur. Overall, DDX43 antibodies contribute to advancing our understanding of its oncogenic mechanisms and therapeutic potential.
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