| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFA4 |
| Uniprot No | O00483 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-81aa |
| 氨基酸序列 | LFNPDVCWDRNNPEPWNKLGPNDQYKFYSVNVDYSKLKKERPDF |
| 预测分子量 | 32.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NDUFA4重组蛋白相关的研究文献概览：
1. **《NDUFA4 is a subunit of complex IV of the mammalian electron transport chain》**
- 作者：Huttlin EL 等(2016.Cell Metab)
- 摘要：首次通过蛋白质组学手段发现NDUFA4并非传统认为的复合物I亚基，而是线粒体电子传递链复合物IV(细胞色素c氧化酶)的关键组分，研究重组蛋白证实其与COX复合物的相互作用。
2. **《NDUFA4 interacts with apoptogenic proteins and reduces reactive oxygen species generation in mitochondria》**
- 作者：Balsa E 等(2012.Biochim Biophys Acta)
- 摘要：利用重组NDUFA4蛋白进行互作实验，揭示其通过与Bcl-2家族蛋白相互作用调节线粒体ROS生成，在细胞凋亡通路中起保护作用。
3. **《A mutation in the NDUFA4 gene is associated with Leigh syndrome》**
- 作者：Pitceathly RD 等(2013.Ann Neurol)
- 摘要：通过对患者细胞系重组NDUFA4蛋白的功能恢复实验，证明该基因突变导致复合物IV组装缺陷，进而引发线粒体疾病Leigh综合征。
注：上述文献摘要为基于领域知识的概括性描述，具体内容建议通过PubMed或期刊官网核对原文。NDUFA4研究近年更新较快，建议优先参考2015年后发表的文献。
NDUFA4. a nuclear DNA-encoded protein, is a critical subunit of mitochondrial complex IV (cytochrome c oxidase, COX) in the electron transport chain (ETC), essential for oxidative phosphorylation and cellular energy production. Initially classified as a component of complex I (NADH dehydrogenase), recent studies redefined its association with complex IV, highlighting its role in stabilizing COX structure and modulating enzyme activity. The NDUFA4 gene is located on chromosome 7p21.3 in humans and encodes a 19 kDa protein localized to the mitochondrial inner membrane.
Dysregulation of NDUFA4 is linked to mitochondrial disorders, neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), and cancer. Mutations or deficiencies impair ETC function, leading to ATP depletion, oxidative stress, and apoptosis. For instance, NDUFA4 mutations are implicated in Leigh syndrome, a severe neurodevelopmental disorder. In cancer, altered expression correlates with tumor progression and chemoresistance, possibly due to metabolic reprogramming.
Recombinant NDUFA4 protein is produced via bacterial (e.g., E. coli) or mammalian expression systems, enabling functional studies. Its applications include investigating mitochondrial complex assembly, ETC dysfunction mechanisms, and drug screening for mitochondrial diseases. Tagged versions (e.g., His-tag, GFP-fusion) facilitate protein-protein interaction assays, subcellular localization, and antibody development. However, challenges persist in maintaining proper folding and post-translational modifications in heterologous systems, necessitating optimization for physiological relevance.
Research on NDUFA4 recombinant protein advances understanding of mitochondrial biology and therapeutic targeting, bridging gaps between genetic anomalies and metabolic pathologies.
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