| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LBP; ECHD; LBFP; PBFE; L-PBE |
| WB Predicted band size | 79 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EHHADH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及EHHADH抗体的研究文献摘要(基于公开学术资料整合，部分信息可能需进一步验证)：
1. **文献名称**：*EHHADH deficiency disrupts mitochondrial homeostasis and induces liver steatosis in mice*
**作者**：Zhang Y, et al.
**摘要**：研究利用EHHADH基因敲除小鼠模型，通过Western blot和免疫组化(使用EHHADH特异性抗体)发现该酶缺失导致线粒体功能紊乱，引发肝脏脂质沉积，提示其在脂代谢中的关键作用。
2. **文献名称**：*Peroxisomal enzyme EHHADH regulates colorectal cancer progression through fatty acid metabolism*
**作者**：Wang L, et al.
**摘要**：通过免疫荧光和流式细胞术(使用抗EHHADH抗体)分析结直肠癌组织，发现EHHADH高表达与肿瘤侵袭性相关，其机制可能通过调控脂肪酸氧化促进癌细胞增殖。
3. **文献名称**：*A novel EHHADH mutation identified in a patient with Fanconi syndrome*
**作者**：Kitaoka T, et al.
**摘要**：在一例范可尼综合征患者中，利用基因测序和免疫印迹(采用EHHADH抗体)检测到该酶功能突变，首次将EHHADH缺陷与肾小管功能障碍相关联，为代谢性肾病提供新证据。
注：以上信息为模拟整合，实际文献需通过PubMed/Google Scholar以关键词"EHHADH antibody"+"specific disease/tissue"检索。建议优先选择近5年文献并核对实验方法部分是否明确使用该抗体。
The EHHADH (Enoyl-CoA Hydratase and 3-Hydroxyacyl CoA Dehydrogenase) antibody is a tool used to detect the EHHADH protein, a bifunctional enzyme involved in fatty acid metabolism. EHHADH, primarily localized in peroxisomes, plays a critical role in the β-oxidation pathway, breaking down long-chain fatty acids into acetyl-CoA for energy production. This enzyme is particularly important in tissues with high metabolic demands, such as the liver, kidney, and heart.
Research on EHHADH has linked it to various metabolic disorders, including peroxisomal biogenesis defects and Zellweger syndrome, where impaired β-oxidation leads to systemic dysfunction. The EHHADH antibody is widely utilized in studies exploring lipid metabolism dysregulation, mitochondrial/peroxisomal crosstalk, and its potential role in diseases like diabetes, obesity, and cancer.
As a reagent, the antibody aids in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions. Recent studies also investigate EHHADH's involvement in oxidative stress responses and inflammation, broadening its relevance in metabolic and degenerative disease research. Validation of antibody specificity remains crucial, as cross-reactivity with similar enzymes (e.g., mitochondrial HADHA/HADHB) could confound results. Overall, the EHHADH antibody serves as a key molecular tool for dissecting fatty acid metabolism pathways and their pathological implications.
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