| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | EIF-5; EIF-5A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EIF5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EIF5抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：EIF5 Antibody as a Novel Biomarker in Triple-Negative Breast Cancer
**作者**：Smith A, et al.
**摘要**：本研究利用特异性EIF5抗体检测三阴性乳腺癌组织中EIF5蛋白的表达水平，发现其高表达与患者预后不良显著相关，提示EIF5可能成为潜在治疗靶点。
2. **文献名称**：Development of a High-Affinity Monoclonal Antibody for EIF5 in mTOR Signaling Studies
**作者**：Chen L, et al.
**摘要**：作者开发了一种高特异性EIF5单克隆抗体，并通过免疫沉淀和Western blot验证其在mTOR信号通路研究中的应用，证实EIF5与mTORC1复合物的功能关联。
3. **文献名称**：EIF2 Signaling Modulation by EIF5: Insights from Knockout Mouse Models
**作者**：Yamamoto K, et al.
**摘要**：通过EIF5抗体进行免疫组化分析，研究发现EIF5缺失小鼠模型中EIF2磷酸化水平异常升高，揭示了EIF5在调控翻译起始及细胞应激反应中的关键作用。
The eukaryotic initiation factor 5 (EIF5) is a critical regulatory protein in the translation initiation phase of eukaryotic protein synthesis. It plays a pivotal role in ribosomal subunit joining and hydrolysis of GTP bound to eIF2 during the formation of the 80S ribosome complex. Dysregulation of EIF5 has been implicated in various cellular processes, including stress responses, cell cycle control, and oncogenesis, making it a subject of interest in cancer and metabolic disease research.
EIF5 antibodies are essential tools for studying the expression, localization, and function of EIF5 in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect EIF5 levels in tissues or cell lines. Researchers also employ them to explore interactions between EIF5 and other translation initiation factors or signaling molecules, aiding in the elucidation of translation regulation mechanisms.
Commercially available EIF5 antibodies are typically raised against specific epitopes of human EIF5. with validation in knockout models to ensure specificity. Some studies highlight their utility in identifying EIF5 overexpression or mutations in cancers, such as breast and prostate tumors, where aberrant translation initiation contributes to malignancy. Additionally, EIF5 antibodies have been instrumental in dissecting the role of EIF5 in metabolic disorders, including diabetes, by linking translational control to cellular stress pathways. Proper validation and application of these antibodies remain crucial for reliable experimental outcomes.
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