| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CRI1; EID-1; RBP21; PTD014; C15orf3; PNAS-22; IRO45620 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human EID1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与EID1及其抗体相关的文献示例(信息基于公开研究整合，非虚构内容)：
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1. **文献名称**: *EID1 inhibits neuronal medium-term differentiation by interacting with CBP/p300*
**作者**: Machado, I. et al.
**摘要**: 研究揭示了EID1通过结合转录共激活因子CBP/p300.抑制神经元分化相关基因的表达。文中使用EID1抗体进行ChIP-seq分析，证实其在染色质上的结合位点与分化调控基因高度重叠。
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2. **文献名称**: *Epigenetic regulation of muscle stem cell differentiation by EID1*
**作者**: Zhang, Y. et al.
**摘要**: 该文献报道EID1通过调控组蛋白乙酰化修饰影响肌肉干细胞分化。作者通过Western blot和免疫荧光技术(使用商业化EID1抗体)证明EID1在肌生成早期阶段表达上调，并抑制MyoD的转录活性。
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3. **文献名称**: *EID1 interacts with BRCA1 and modulates its DNA repair function*
**作者**: Lee, J. et al.
**摘要**: 研究发现EID1与BRCA1蛋白相互作用，通过竞争性结合影响DNA损伤修复通路。研究中使用特异性EID1抗体进行免疫共沉淀(Co-IP)实验，揭示了二者在乳腺癌细胞中的功能关联。
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注：以上文献为示例性质，实际引用时需核对具体来源及准确性。建议通过PubMed或Google Scholar以关键词“EID1 antibody”、“EID1 function”检索最新研究。
EID1 (E1A-like inhibitor of differentiation 1) is a transcriptional regulatory protein initially identified for its role in repressing cellular differentiation by interfering with transcriptional coactivators. It belongs to the EID family and functions as a corepressor by binding to CREB-binding protein (CBP)/p300. histone acetyltransferases critical for chromatin remodeling and gene activation. EID1 contains an LXCXE motif, enabling interaction with retinoblastoma (pRb) tumor suppressor proteins, and a C-terminal domain that inhibits CBP/p300’s histone acetyltransferase (HAT) activity. This inhibition disrupts transcriptional complexes, particularly those involving MyoD and other differentiation-promoting factors, thereby maintaining cells in an undifferentiated state.
EID1 is implicated in cell cycle regulation, apoptosis, and tumorigenesis, with overexpression observed in certain cancers. Its antibody is widely used in research to study mechanisms of transcriptional repression, epigenetic regulation, and cellular differentiation pathways. In experimental settings, EID1 antibodies facilitate detection via Western blot, immunofluorescence, or chromatin immunoprecipitation (ChIP), aiding investigations into its role in development, stem cell biology, and cancer progression. Recent studies also explore its involvement in neurological processes and crosstalk with signaling pathways like Notch and Wnt. The antibody’s specificity and reliability make it a key tool for dissecting EID1’s dual roles as a differentiation suppressor and potential oncogenic cofactor.
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