| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KNSL3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human KIF25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于KIF25相关研究的示例性参考文献(部分信息可能需根据实际文献调整，建议通过学术数据库核实)：
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1. **标题**: "KIF25 promotes colorectal cancer progression by regulating microtubule dynamics and cytokinesis"
**作者**: Tanaka H, et al.
**摘要**: 研究揭示KIF25在结直肠癌中过表达，通过调控微管动力学促进肿瘤细胞增殖和迁移。使用KIF25抗体进行免疫组化，发现其高表达与患者预后不良相关。
2. **标题**: "Functional characterization of KIF25 in mitotic spindle organization and chromosomal segregation"
**作者**: Smith JL, et al.
**摘要**: 通过siRNA敲低和KIF25抗体免疫荧光成像，证实KIF25参与有丝分裂纺锤体组装及染色体分离，缺失导致细胞分裂异常和基因组不稳定性。
3. **标题**: "Development of a monoclonal antibody against KIF25 for diagnostic applications in pancreatic cancer"
**作者**: Chen R, et al.
**摘要**: 报道一种高特异性抗KIF25单克隆抗体的开发，验证其在胰腺癌组织中的诊断潜力，并通过Western blot和流式细胞术确认其灵敏度。
4. **标题**: "KIF25 interaction with cytoskeletal components and implications in neuromuscular disorders"
**作者**: Müller S, et al.
**摘要**: 利用KIF25抗体进行共免疫沉淀实验，发现其与肌动蛋白结合蛋白的相互作用，提示KIF25功能障碍可能与神经肌肉疾病相关。
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**注意**：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索确认。若研究较少，可扩展至KIF家族其他成员或抗体开发技术文献。
The KIF25 antibody is a research tool designed to detect and study kinesin family member 25 (KIF25), a protein belonging to the kinesin superfamily. Kinesins are microtubule-dependent motor proteins involved in intracellular transport, mitosis, and cell signaling. KIF25. specifically, is a relatively less characterized member of this family. It contains a conserved kinesin motor domain at its N-terminus, enabling ATP-dependent movement along microtubules, and a divergent C-terminal region that may mediate interactions with cargo molecules or regulatory partners.
KIF25 is implicated in cellular processes such as organelle trafficking, spindle dynamics during cell division, and possibly cancer progression. Studies suggest its overexpression in certain cancers, including colorectal and breast cancer, may correlate with tumor aggressiveness. The KIF25 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate its expression, localization, and functional roles in both physiological and pathological contexts.
Validation of KIF25 antibodies typically involves testing specificity via knockout cell lines or siRNA-mediated knockdown. Researchers rely on these antibodies to explore KIF25's contributions to cellular dynamics and disease mechanisms, though its precise biological functions and regulatory pathways remain under active investigation.
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