Rabbit Polyclonal FLI1 Antibody

The FLI1 antibody is a crucial tool in diagnostic and research pathology, primarily targeting the FLI1 protein encoded by the *FLI1* gene. FLI1. a member of the ETS transcription factor family, plays roles in hematopoiesis, vasculogenesis, and oncogenesis. It regulates gene expression by binding to DNA sequences containing GGAA/T motifs. Aberrant FLI1 expression is implicated in several malignancies, most notably Ewing sarcoma, where a chromosomal translocation t(11;22)(q24;q12) generates the *EWS::FLI1* fusion oncoprotein, present in 85-90% of cases. FLI1 antibodies are widely used in immunohistochemical (IHC) panels to distinguish Ewing sarcoma from other small round cell tumors (e.g., rhabdomyosarcoma, lymphoma) due to its nuclear expression in tumor cells. Additionally, FLI1 is expressed in vascular endothelial cells and vascular neoplasms (e.g., angiosarcoma, epithelioid hemangioendothelioma), making the antibody useful in diagnosing vascular tumors. However, its specificity requires careful interpretation, as FLI1 can also be detected in lymphomas, melanomas, and some carcinomas. Research applications include studying FLI1's role in tumor biology, hematologic disorders, and vascular development. Cross-reactivity with other ETS family proteins and variable staining intensity across tissue types remain challenges, emphasizing the need for validation with controls and complementary molecular tests.