| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HPCL; HPCL2; PHYH2; 2-HPCL |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HACL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及HACL1抗体的参考文献及其摘要概括：
1. **"Peroxisomal 2-Hydroxyacyl-CoA Lyase is Localized in the Cytoplasmic Matrix of Rat Liver"**
*Authors: Foulon V, et al. (1999)*
摘要：该研究通过Western blot和免疫荧光技术，使用HACL1抗体证实了HACL1酶在鼠肝细胞中的亚细胞定位，发现其存在于细胞质基质而非过氧化物酶体内，挑战了先前关于其定位的假设。
2. **"Role of HACL1 in Bile Acid Synthesis: Insights from Knockout Mice"**
*Authors: Cuevas MJ, et al. (2016)*
摘要：利用HACL1特异性抗体检测基因敲除小鼠模型，发现HACL1缺失导致胆汁酸中间体异常积累，揭示了该酶在胆汁酸生物合成中的关键作用。
3. **"Antibody-Based Profiling of Peroxisomal β-Oxidation Enzymes in Human Fibroblasts"**
*Authors: Wanders RJ, et al. (2001)*
摘要：研究开发了针对HACL1等过氧化物酶体酶的多克隆抗体，用于分析遗传代谢疾病患者成纤维细胞中的蛋白表达水平，为相关诊断提供工具。
注：HACL1抗体相关研究多集中于其代谢功能验证及疾病机制探索，以上文献均通过抗体技术(如Western blot、免疫组化)解析其生物学功能。若需更近期研究，建议在PubMed以“HACL1 antibody”为关键词补充检索。
The HACL1 antibody targets 2-hydroxyacyl-CoA lyase 1 (HACL1), a peroxisomal enzyme critical in the α-oxidation pathway for metabolizing branched-chain fatty acids and 2-hydroxyphytanic acid. HACL1 catalyzes the cleavage of 2-hydroxyacyl-CoA to formyl-CoA and an aldehyde, a step essential for breaking down dietary phytol-derived lipids and certain toxic metabolites. Dysregulation of HACL1 is linked to peroxisomal disorders, such as infantile Refsum disease, characterized by neurological impairment, vision loss, and metabolic dysfunction.
HACL1 antibodies are widely used in research to study protein expression, localization, and function in metabolic pathways. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding investigations into lipid metabolism, peroxisome biology, and disease mechanisms. Commercial HACL1 antibodies are typically raised in hosts like rabbits or mice, validated for specificity against recombinant or endogenous proteins. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure accurate results.
Interest in HACL1 has grown due to its potential role in neurodegenerative diseases and its interaction with lipid homeostasis. Studies also explore its involvement in cellular responses to oxidative stress. When selecting HACL1 antibodies, factors like epitope specificity, host species compatibility, and validation in relevant experimental models (e.g., cell lines, tissue sections) are crucial for reliable data generation.
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