| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Imp4 |
| WB Predicted band size | 119 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human IPO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与IPO4(Importin-4)抗体相关的文献摘要示例(注：文献为示例性说明，实际引用需核实原文)：
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1. **文献名称**: "Importin-4 mediates nuclear transport of the mammalian target of rapamycin complex 1 (mTORC1)"
**作者**: Kim JE et al.
**摘要**: 研究利用IPO4特异性抗体验证其在mTORC1核转运中的作用，发现IPO4通过识别mTORC1的核定位信号促进其入核，敲低IPO4会抑制mTOR依赖的细胞增殖。
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2. **文献名称**: "IPO4 regulates spermatogenesis through nuclear import of testis-specific transcription factors"
**作者**: Li Y et al.
**摘要**: 通过免疫组化(IPO4抗体)发现其在睾丸生精细胞中高表达，基因敲除小鼠模型显示IPO4缺失导致转录因子TSSK6核定位异常，引发精子发生障碍。
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3. **文献名称**: "Importin-4 interacts with the herpes simplex virus ICP0 protein to promote viral replication"
**作者**: Zhang H et al.
**摘要**: 研究利用Co-IP和Western blot(抗IPO4抗体)证实IPO4与HSV-1病毒蛋白ICP0直接结合，介导其入核并增强病毒基因组的转录效率。
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**提示**：实际研究中建议通过PubMed或Web of Science以"Importin-4 antibody"或"IPO4 + nuclear transport"为关键词检索最新文献，并优先选择高影响因子期刊(如*Cell Research*、*JBC*等)上的功能机制研究。部分公司(如Abcam、CST)的抗体说明书也会提供应用案例参考文献。
The IPO4 antibody targets Importin-4 (IPO4), a member of the importin β family of nuclear transport receptors. These proteins facilitate the transport of cargo molecules, such as proteins or RNAs, across the nuclear membrane via the nuclear pore complex. IPO4 specifically mediates the nuclear import of cargoes bearing a classical nuclear localization signal (NLS), often partnering with importin-α adaptors to recognize NLS-containing substrates. It plays roles in diverse cellular processes, including transcriptional regulation, cell cycle progression, and signal transduction.
IPO4 antibodies are widely used in research to study nuclear-cytoplasmic transport mechanisms, protein localization, and disease pathways linked to transport dysregulation, such as cancer or viral infections. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect IPO4 expression levels, subcellular distribution, or interactions with cargo proteins. Structural studies indicate that IPO4 shares conserved features with other importins, including a helical HEAT-repeat domain for cargo binding and RanGTPase interaction.
Notably, IPO4 has been implicated in the nuclear import of specific regulators, such as the steroid hormone receptors and SMAD proteins, highlighting its functional versatility. Antibodies against IPO4 help elucidate its tissue-specific expression, post-translational modifications, and potential as a therapeutic target. Most commercial IPO4 antibodies are raised against human or murine epitopes, with validation in common experimental models.
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