| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BUB3L; hBUB3 |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human BUB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BUB3抗体的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**：*BUB3 is essential for the assembly of a functional Mad2–Cdc20 complex in human cells*
**作者**：Taylor SS, et al.
**摘要**：本研究利用特异性BUB3抗体通过免疫共沉淀和RNA干扰技术，揭示了BUB3在纺锤体检查点中调控Mad2-Cdc20复合体形成的关键作用，并证实其缺失导致染色体分离异常。
2. **文献名称**：*Development of a monoclonal antibody against human BUB3 for cancer biomarker screening*
**作者**：Chen L, et al.
**摘要**：研究者开发了一种高特异性抗人BUB3单克隆抗体，通过免疫组化分析发现BUB3在结直肠癌组织中的表达显著上调，提示其可能作为癌症诊断的潜在生物标志物。
3. **文献名称**：*BUB3 phosphorylation regulates kinetochore-microtubule attachment in yeast*
**作者**：Hardwick KG, et al.
**摘要**：通过BUB3抗体进行Western blot和活细胞成像，发现BUB3的磷酸化修饰对其在酵母中调控微管-动粒连接的稳定性至关重要，缺陷会导致染色体错误分离。
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注：以上文献为示例，实际引用时请核实真实文献信息及摘要细节。如需具体文献，建议通过PubMed或Google Scholar检索关键词“BUB3 antibody”获取最新研究。
BUB3 antibodies are essential tools for studying the spindle assembly checkpoint (SAC), a critical surveillance mechanism ensuring accurate chromosome segregation during mitosis. BUB3. a conserved SAC protein, forms complexes with BUB1 and BUBR1 to monitor microtubule-kinetochore attachment. It acts as a scaffold, facilitating protein interactions and signal transduction that delay anaphase until all chromosomes achieve proper bipolar attachment. Dysregulation of BUB3 is linked to chromosomal instability, aneuploidy, and cancer progression.
Researchers use BUB3 antibodies to investigate its expression levels, subcellular localization, and interactions in normal and pathological contexts, such as tumorigenesis or chemotherapy resistance. These antibodies are widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity validation via knockout/knockdown controls is crucial, as off-target reactivity may compromise data reliability. Recent studies also explore BUB3's non-mitotic roles, including DNA damage response and immune regulation, expanding its relevance beyond cell cycle research. Commercial BUB3 antibodies are typically raised against recombinant human protein fragments, with rabbit and mouse being common host species. Proper antibody selection and experimental optimization remain key to elucidating BUB3's multifaceted functions in health and disease.
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