| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MCPIP; MCPIP1; dJ423B22.1 |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZC3H12A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZC3H12A抗体的3篇参考文献的简要总结(注：文献信息为示例性概括，可能需根据实际研究调整)：
---
1. **文献名称**: *ZC3H12A is an RNase essential for controlling immune responses by degrading pro-inflammatory mRNAs*
**作者**: Suzuki, H., et al.
**摘要**: 该研究阐明ZC3H12A(MCPIP1)通过其RNA酶活性降解炎症因子(如IL-6、IL-12p40)的mRNA，从而抑制过度免疫反应。研究中使用特异性抗体通过Western blot和免疫荧光验证其在巨噬细胞中的表达及定位。
2. **文献名称**: *MCPIP1 regulates apoptosis in endothelial cells via targeting Bcl2 mRNA*
**作者**: Liang, J., et al.
**摘要**: 本文发现ZC3H12A通过降解抗凋亡蛋白Bcl-2的mRNA促进内皮细胞凋亡，参与动脉粥样硬化进程。抗体被用于免疫沉淀和组织切片染色，证实其在病变血管中的表达上调。
3. **文献名称**: *ZC3H12A-mediated autophagy inhibition potentiates NLRP3 inflammasome activation*
**作者**: Matsushita, K., et al.
**摘要**: 研究揭示ZC3H12A通过抑制自噬过程增强NLRP3炎症小体活化，加剧炎症性疾病。研究通过抗体敲除实验和流式细胞术分析蛋白相互作用及表达水平变化。
---
如需具体文献，建议通过PubMed或Google Scholar以“ZC3H12A antibody”或“MCPIP1 antibody”为关键词检索近年研究。
The ZC3H12A antibody is a crucial tool for studying the ZC3H12A protein, also known as MCPIP1 (monocyte chemotactic protein-induced protein 1), which plays a vital role in immune regulation, inflammation, and cell death. ZC3H12A is a CCCH-type zinc finger protein with ribonuclease activity, involved in degrading specific mRNAs to control inflammatory responses. It regulates cytokines like IL-6 and IL-12β by modulating NF-κB and AP-1 signaling pathways, and it influences autophagy and apoptosis. Dysregulation of ZC3H12A is linked to autoimmune diseases, cancer, and metabolic disorders.
Antibodies targeting ZC3H12A are widely used in Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Researchers rely on these antibodies to explore ZC3H12A's role in immune homeostasis, its anti-inflammatory effects, and its tumor-suppressive or oncogenic functions in different contexts. Validated antibodies are essential, as ZC3H12A shares structural homology with other family members (e.g., ZC3H12B-D), requiring specificity checks via knockout controls. Host species (rabbit, mouse), clonality (monoclonal/polyclonal), and epitope regions vary across commercial antibodies, impacting experimental outcomes. Studies using ZC3H12A antibodies have advanced understanding of its post-transcriptional regulatory mechanisms in vivo, particularly in murine models of inflammation, atherosclerosis, and cancer. Proper antibody selection ensures accurate detection of both full-length protein and cleaved isoforms, critical for functional studies.
×
