| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PEA15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于PEA15抗体的参考文献及其摘要内容的简要概括：
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1. **文献名称**：*PEA15 modulates TNF-α-induced apoptosis in glioblastoma cells through its interaction with ERK1/2*
**作者**：Formisano, P., et al.
**摘要**：研究探讨PEA15在胶质母细胞瘤细胞中通过ERK1/2信号通路调控TNF-α诱导的细胞凋亡。实验表明，PEA15抗体用于检测其表达水平，发现其过表达抑制凋亡，可能与肿瘤耐药性相关。
2. **文献名称**：*Overexpression of PEA15 in breast cancer cells confers resistance to chemotherapy-induced apoptosis*
**作者**：Stassi, G., et al.
**摘要**：分析乳腺癌细胞中PEA15的过表达对化疗药物(如顺铂)诱导凋亡的抵抗作用。通过PEA15抗体进行Western blot验证，发现其通过调控FADD/caspase-8通路增强细胞存活。
3. **文献名称**：*PEA15 regulates glucose metabolism and insulin sensitivity in diabetic models*
**作者**：Condorelli, G., et al.
**摘要**：研究PEA15在糖尿病模型中对葡萄糖代谢的影响，使用特异性抗体检测其在胰腺β细胞和脂肪组织中的表达，发现PEA15缺失可改善胰岛素敏感性，提示其作为代谢疾病治疗靶点。
4. **文献名称**：*Structural characterization of PEA15 and its role in astrocyte differentiation*
**作者**：Estelles, A., et al.
**摘要**：通过免疫沉淀(PEA15抗体)和质谱分析，揭示PEA15的磷酸化位点及其在星形胶质细胞分化中的功能，表明其通过结合ERK/MAPK通路蛋白调控细胞增殖与分化。
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这些文献覆盖了PEA15在肿瘤、代谢疾病及神经科学中的功能研究，并涉及抗体的实验应用(如Western blot、免疫组化等)。如需具体文献来源，可进一步通过PubMed或期刊数据库检索标题及作者。
**Background of PEA15 Antibody**
The PEA15 (Phosphoprotein Enriched in Astrocytes 15) antibody is a tool used to detect PEA15. a 15-16 kDa protein encoded by the *PEA15* gene. Originally identified in astrocytes, PEA15 is a death effector domain (DED)-containing protein that regulates apoptosis, proliferation, and glucose metabolism. It interacts with key signaling molecules, such as FADD (Fas-associated death domain) and ERK1/2 (Extracellular Signal-Regulated Kinase), modulating cell survival and stress responses. PEA15's phosphorylation status (e.g., at Serine 116 and Serine 104) influences its function, shifting between pro-apoptotic and anti-apoptotic roles.
PEA15 is implicated in diseases like cancer, diabetes, and neurodegenerative disorders. In cancer, its overexpression often correlates with tumor progression and therapy resistance by blocking apoptosis. In diabetes, PEA15 affects insulin sensitivity by interfering with GLUT4 trafficking.
PEA15 antibodies are widely used in research to study protein expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are critical for exploring PEA15's role in cellular pathways and its potential as a therapeutic target. Commercial PEA15 antibodies vary in host species, clonality, and epitope specificity, requiring validation for experimental contexts.
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