| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HST, KFGF, HST-1, HSTF1, K-FGF, HBGF-4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FGF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3-4条关于PHLPP1抗体的参考文献的简要列举(信息基于公开研究，可能需核对原文准确性)：
---
1. **文献名称**: "PHLPP1: A Tumor Suppressor Protein Phosphatase that Directly Inactivates AKT"
**作者**: Gao T. et al.
**摘要**: 该研究首次鉴定了PHLPP1作为AKT的直接磷酸酶，通过特异性抗体验证其在多种癌细胞系中的表达，并证明其缺失导致AKT过度激活，促进肿瘤生长。
---
2. **文献名称**: "The PP2A/PHLPP1 Axis Regulates Apoptosis in Cancer via AKT Inactivation"
**作者**: Brognard J. et al.
**摘要**: 研究利用PHLPP1抗体进行免疫沉淀和Western blot分析，揭示了PHLPP1通过去磷酸化AKT诱导癌细胞凋亡的机制，为癌症治疗提供潜在靶点。
---
3. **文献名称**: "PHLPP1 Deficiency Enhances Neuronal Survival in Ischemic Stroke Models"
**作者**: Siuta M.A. et al.
**摘要**: 通过PHLPP1抗体在小鼠脑组织中的免疫组化分析，发现PHLPP1敲除可减少缺血性神经元死亡，表明其在神经退行性疾病中的调控作用。
---
4. **文献名称**: "Targeting PHLPP1 Improves Insulin Signaling in Diabetic Mouse Models"
**作者**: Chen M. et al.
**摘要**: 研究使用PHLPP1抗体检测糖尿病模型中的蛋白表达，证实抑制PHLPP1可恢复胰岛素信号通路活性，改善糖代谢异常。
---
**注意**：以上内容为示例性概括，具体文献需通过PubMed、Google Scholar等平台检索确认。若需实际引用，请核对作者、期刊及年份的准确性。
PHLPP1 (Pleckstrin Homology Domain Leucine-Rich Repeat Protein Phosphatase 1) is a serine/threonine phosphatase that plays a critical role in regulating cellular signaling pathways, particularly the PI3K/Akt and Ras/Raf/MEK/ERK cascades. It functions as a tumor suppressor by dephosphorylating and inactivating key kinases like Akt and PKC, thereby modulating cell survival, proliferation, and apoptosis. Dysregulation of PHLPP1 has been implicated in cancer, neurodegenerative disorders, and metabolic diseases, with its downregulation often linked to poor prognosis and therapy resistance.
Antibodies targeting PHLPP1 are essential tools for studying its expression, localization, and interactions in various biological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate PHLPP1's role in health and disease. Researchers utilize them to assess tissue-specific expression patterns, monitor post-translational modifications, or evaluate correlations between PHLPP1 levels and clinical outcomes. Due to structural similarities between PHLPP1 and its isoform PHLPP2. antibody specificity is rigorously validated to avoid cross-reactivity. Recent studies also employ PHLPP1 antibodies in therapeutic exploration, such as identifying biomarkers or developing strategies to restore PHLPP1 function in cancers. Proper validation using knockout controls or siRNA-mediated silencing remains critical for experimental accuracy.
×
