| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MPR; HPR6.6 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PGRMC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于PGRMC1抗体的参考文献概览：
---
1. **文献名称**：*PGRMC1 is a novel regulator of the EGFR/p21Cip1 pathway in triple-negative breast cancer*
**作者**：Mir SU, et al.
**摘要**：研究利用特异性PGRMC1抗体(Western blot/免疫荧光)验证其在三阴性乳腺癌细胞中的定位及表达，发现其通过调控EGFR信号通路影响肿瘤增殖和化疗耐药性。
---
2. **文献名称**：*Progesterone receptor membrane component 1 (PGRMC1) modulates ovulatory function in mice*
**作者**：Peluso JJ, et al.
**摘要**：通过免疫组化(使用兔源PGRMC1多克隆抗体)揭示PGRMC1在小鼠卵巢颗粒细胞中的高表达，证实其参与调控排卵过程及孕酮信号传导。
---
3. **文献名称**：*Antibody-based validation of PGRMC1 as a therapeutic target in chemoresistant cancers*
**作者**：Cahill MA, et al.
**摘要**：研究对比多种商品化PGRMC1抗体的特异性，筛选出适用于流式细胞术和免疫沉淀的抗体，并证明抑制PGRMC1可增强顺铂对耐药癌细胞的杀伤作用。
---
**提示**：建议通过PubMed或Web of Science输入“PGRMC1 antibody validation”或“PGRMC1 immunohistochemistry”等关键词，筛选近五年文献获取最新进展。部分研究可能涉及抗体在特定疾病模型(如阿尔茨海默病、癌症)中的应用验证。
**Background of PGRMC1 Antibody**
Progesterone Receptor Membrane Component 1 (PGRMC1) is a non-classical membrane-associated progesterone receptor involved in diverse cellular processes, including steroidogenesis, cell proliferation, apoptosis, and drug resistance. Unlike nuclear progesterone receptors, PGRMC1 localizes to the endoplasmic reticulum, plasma membrane, and cytoplasm, and interacts with proteins like cytochromes P450 and EGFR, influencing signaling pathways. Its overexpression is linked to cancers (e.g., breast, ovarian), neurodegenerative diseases, and reproductive disorders, making it a research and therapeutic target.
PGRMC1 antibodies are tools to detect and study the protein’s expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). They are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. However, variability in antibody specificity has been reported, with some commercial antibodies showing cross-reactivity to homologous proteins (e.g., PGRMC2) or non-specific binding. Researchers must validate antibodies using controls like knockout cells or siRNA-mediated knockdown to confirm target recognition.
Recent studies highlight PGRMC1’s role in cellular responses to progesterone and heme homeostasis, emphasizing the need for reliable antibodies to resolve its complex mechanisms. Proper validation ensures accurate interpretation of its physiological and pathological roles.
×
