| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MRB; AOVD3; ECSM4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ROBO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ROBO4抗体的代表性文献概览：
1. **"ROBO4 maintains vascular integrity by suppressing endothelial endocytosis"**
- 作者：Jones CA, et al.
- 摘要：发现ROBO4抗体可特异性阻断内皮细胞的内吞作用，揭示ROBO4通过稳定细胞连接减少血管渗漏，为治疗血管水肿提供靶点。
2. **"Anti-ROBO4 immunotherapy inhibits angiogenesis in colorectal cancer models"**
- 作者：Zhang L, et al.
- 摘要：开发人源化ROBO4单克隆抗体，证明其通过阻断VEGF协同信号通路抑制肿瘤血管生成，显著降低小鼠结直肠癌转移率。
3. **"ROBO4 as a biomarker of endothelial dysfunction in diabetic retinopathy"**
- 作者：Kim S, et al.
- 摘要：使用ROBO4抗体检测发现糖尿病患者视网膜血管中ROBO4表达异常下调，提示其可作为早期视网膜病变的诊断标志物。
*注：以上为示例性概括，实际文献需通过PubMed/Web of Science以"ROBO4"+"antibody"为关键词检索获取。近年研究多聚焦于ROBO4在血管疾病中的双重调控机制及抗体药物开发。*
**Background of ROBO4 Antibody**
ROBO4 (Roundabout homolog 4) is a transmembrane receptor belonging to the Roundabout (Robo) family, which plays critical roles in vascular development and angiogenesis. Primarily expressed in endothelial cells, ROBO4 regulates cell migration, adhesion, and vascular stabilization by interacting with its ligands, such as Slit proteins and other non-Slit ligands (e.g., UNC5B). Unlike other Robo receptors, ROBO4 is largely restricted to the vascular system and is involved in maintaining endothelial barrier integrity and modulating pathological angiogenesis.
ROBO4 gained attention for its dual role in both promoting and inhibiting angiogenesis, depending on cellular context and ligand interactions. Studies highlight its importance in embryonic vascular development and its re-emergence in adult endothelial cells during diseases like cancer, diabetic retinopathy, and inflammatory disorders. ROBO4 signaling often intersects with pathways like VEGF and Wnt, making it a potential therapeutic target.
ROBO4 antibodies are essential tools for detecting ROBO4 expression in vascular tissues, studying its function in angiogenesis assays, and exploring its therapeutic potential. These antibodies enable researchers to investigate ROBO4's role in endothelial cell behavior, vascular permeability, and disease mechanisms. Recent research also explores ROBO4-targeted therapies to normalize tumor vasculature or block pathological angiogenesis, underscoring its clinical relevance in vascular-related diseases.
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