| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ARA54; HFB30; TRIAD2; HRIHFB2038 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RNF14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RNF14抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*RNF14 is a novel regulator of Wnt signaling in neuroblastoma*
**作者**：Smith A, et al.
**摘要**：该研究利用RNF14抗体通过免疫共沉淀技术，揭示了RNF14在神经母细胞瘤中通过调控Wnt/β-catenin通路促进肿瘤进展的机制，证实其与β-catenin的泛素化修饰相关。
2. **文献名称**：*Role of RNF14 in the DNA damage response and hormone receptor regulation*
**作者**：Chen L, et al.
**摘要**：研究通过Western blot和免疫荧光技术(使用RNF14特异性抗体)，证明RNF14参与DNA损伤修复过程，并作为雄激素受体的共激活因子，影响前列腺癌细胞的激素依赖性生长。
3. **文献名称**：*RNF14 interacts with p53 and modulates its transcriptional activity*
**作者**：Garcia-Reyes M, et al.
**摘要**：该文献利用RNF14抗体进行ChIP实验，发现RNF14与p53蛋白结合，通过泛素化修饰调控p53的稳定性及其靶基因表达，进而影响细胞凋亡和肿瘤抑制功能。
注：以上文献信息为示例性内容，实际引用需根据具体论文核实。建议通过PubMed或Web of Science检索最新相关研究。
The RNF14 antibody targets the RING finger protein 14 (RNF14), a member of the E3 ubiquitin ligase family characterized by a conserved RING finger domain that facilitates protein-protein interactions and ubiquitination. RNF14 plays a multifaceted role in cellular processes, including transcriptional regulation, DNA damage repair, and hormone signaling pathways, particularly interacting with androgen and estrogen receptors. It is implicated in modulating nuclear receptor activity by promoting ligand-dependent degradation or stabilization of target proteins, linking it to hormone-related cancers like prostate and breast cancer.
Antibodies against RNF14 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation to explore RNF14’s involvement in oncogenesis, metabolic regulation, and cellular stress responses. Commercial RNF14 antibodies are typically validated for specificity and cross-reactivity across human, mouse, and rat samples. Research utilizing these antibodies has highlighted RNF14’s dual roles as a potential tumor suppressor or promoter, depending on cellular context, making it a focus for therapeutic targeting. Recent studies also suggest its interaction with viral proteins, expanding its relevance to infectious disease research.
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