| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMRAL1 |
| Uniprot No | Q9HBL8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMVDKKL VVVFGGTGAQ GGSVARTLLE DGTFKVRVVT RNPRKKAAKE LRLQGAEVVQ GDQDDQVIME LALNGAYATF IVTNYWESCS QEQEVKQGKL LADLARRLGL HYVVYSGLEN IKKLTAGRLA AAHFDGKGEV EEYFRDIGVP MTSVRLPCYF ENLLSHFLPQ KAPDGKSYLL SLPTGDVPMD GMSVSDLGPV VLSLLKMPEK YVGQNIGLST CRHTAEEYAA LLTKHTRKVV HDAKMTPEDY EKLGFPGARD LANMFRFYAL RPDRDIELTL RLNPKALTLD QWLEQHKGDF NLL |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NMRAL1重组蛋白的3篇参考文献(模拟示例，基于领域知识推测内容，具体文献需自行验证)：
1. **文献名称**: *"NMRAL1 functions as a redox-sensitive regulator of antioxidant response through interaction with thioredoxin-1"*
**作者**: Zhang Y, et al.
**摘要**: 研究利用重组NMRAL1蛋白，揭示了其通过结合硫氧还蛋白-1(Trx-1)调控细胞氧化还原稳态的机制，证明其在抗氧化应激中的关键作用。
2. **文献名称**: *"Structural and functional characterization of recombinant NMRAL1 in bacterial stress adaptation"*
**作者**: Lee S, Kim JH.
**摘要**: 通过大肠杆菌表达系统纯化NMRAL1重组蛋白，解析其晶体结构，发现其通过结合NADH/NAD+参与细菌代谢应激响应通路。
3. **文献名称**: *"NMRAL1 promotes tumor metastasis via HIF-1α stabilization in hypoxic environments"*
**作者**: Wang L, et al.
**摘要**: 利用重组人源NMRAL1蛋白进行体外实验，证实其通过稳定HIF-1α增强肿瘤细胞在缺氧条件下的侵袭转移能力，为癌症治疗提供新靶点。
注：以上内容为假设性示例，实际文献需通过PubMed/Web of Science等平台检索确认。建议结合关键词 **"NMRAL1 recombinant protein"** 或 **"NMRAL1 purification"** 查找最新研究。
NMRAL1 (NmrA-like family domain-containing protein 1), also known as HSCARG or C9orf95. is a redox-sensitive regulatory protein involved in cellular stress responses and metabolic homeostasis. It belongs to the NmrA family of transcriptional regulators and contains a conserved RING finger-like domain, enabling interactions with nucleic acids and proteins. NMRAL1 is ubiquitously expressed in human tissues and localizes predominantly to the nucleus and cytoplasm.
Functionally, NMRAL1 acts as a sensor of intracellular NAD⁺/NADH ratios, linking cellular redox status to transcriptional and signaling pathways. It modulates NF-κB activity by binding to IKKβ, suppressing inflammation under oxidative stress. Additionally, NMRAL1 interacts with argininosuccinate synthase (ASS1), regulating nitric oxide production and urea cycle intermediates. Its role in DNA damage repair, cell cycle regulation, and apoptosis has also been reported, particularly in cancer contexts where it may act as a tumor suppressor.
Recombinant NMRAL1 protein is produced using expression systems like *E. coli* or mammalian cells (e.g., HEK293), often tagged with His or GST for purification via affinity chromatography. This engineered protein retains functional domains, enabling studies on its structure, post-translational modifications (e.g., phosphorylation), and interactions. Researchers use it to dissect mechanisms in oxidative stress-related diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Its applications extend to drug screening, biomarker discovery, and elucidating NAD⁺-dependent pathways in aging and immunity.
Current research focuses on NMRAL1's dual roles in redox balance and disease pathogenesis, highlighting its potential as a therapeutic target or diagnostic tool in conditions linked to oxidative stress and metabolic dysregulation.
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