| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NME1 |
| Uniprot No | P15531 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-152aa |
| 氨基酸序列 | ANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE |
| 预测分子量 | 44.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NME1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Structural and functional insights into the interaction of human NME1 with nucleotide substrates*
**作者**: Jané, P., et al.
**摘要**: 本研究通过重组表达纯化人源NME1蛋白，利用X射线晶体学解析其三维结构，揭示了NME1与GDP/ATP的结合模式，并验证其核苷二磷酸激酶(NDPK)活性机制，为靶向NME1的肿瘤治疗提供结构基础。
2. **文献名称**: *Recombinant NME1 suppresses metastasis in vitro by modulating cytoskeletal dynamics*
**作者**: Steeg, P.S., et al.
**摘要**: 文章通过大肠杆菌表达重组NME1蛋白，体外实验表明其通过调控微管聚合和Rho-GTPase信号通路抑制肿瘤细胞迁移，支持NME1作为转移抑制因子的功能。
3. **文献名称**: *Biochemical characterization of recombinant NME1/GAPDH complex in DNA repair*
**作者**: Aguiar, R.C.T., et al.
**摘要**: 研究重组表达并纯化NME1与GAPDH的复合物，发现两者协同参与氧化损伤后的DNA修复过程，揭示了NME1在基因组稳定性中的新功能。
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**备注**：以上文献为示例，实际文献需通过PubMed或Google Scholar检索关键词“NME1 recombinant protein”“NM23-H1 purification”等获取最新研究。如需具体文献，建议补充研究领域方向(如结构、功能或疾病关联)。
NME1 (Non-Metastatic Protein 1), also known as NM23-H1. is a member of the NME/NM23 nucleoside diphosphate (NDP) kinase family. It was initially identified as a metastasis suppressor gene due to its reduced expression in highly metastatic cancer cell lines. The protein plays multifaceted roles in cellular processes, including nucleotide metabolism, signal transduction, and maintenance of genomic stability. Its enzymatic activity involves transferring phosphate groups from nucleoside triphosphates to diphosphates, critical for balancing nucleotide pools essential for DNA/RNA synthesis and energy transfer. Beyond this, NME1 interacts with proteins involved in cytoskeletal dynamics, transcription regulation, and apoptosis, linking it to tumor progression and metastasis inhibition.
Recombinant NME1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, biologically active forms for research and therapeutic exploration. Its recombinant version retains key functional properties, such as NDP kinase activity and binding capabilities to partners like glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or telomeric repeat-binding factor 2 (TRF2). Studies leveraging recombinant NME1 have clarified its anti-metastatic mechanisms, including suppression of cell migration, invasion, and induction of differentiation. Additionally, it has been implicated in DNA repair pathways and exosome-mediated intercellular communication.
Clinically, NME1 expression levels correlate with prognosis in cancers like breast, liver, and melanoma, driving interest in its therapeutic potential. Recombinant NME1 serves as a tool to study metastasis biology, screen anticancer drugs, and develop protein-based therapies. Ongoing research explores its role in non-cancer contexts, including developmental biology and neurodegenerative diseases, underscoring its broad biological significance.
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