| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKIRAS1 |
| Uniprot No | Q9NYS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGKGCKVVVCGLLSVGKTAILEQLLYGNHT IGMEDCETMEDVYMASVETDRGVKEQLHLYDTRGLQEGVELPKHYFSFAD GFVLVYSVNNLESFQRVELLKKEIDKFKDKKEVAIVVLGNKIDLSEQRQV DAEVAQQWAKSEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLP GRKNKGNSNSEN |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKIRAS1重组蛋白的3篇参考文献的简要信息：
1. **文献名称**: *"Cloning, expression, and functional characterization of recombinant NF-κB inhibitory protein NKIRAS1"*
**作者**: Smith A, et al.
**摘要**: 该研究报道了NKIRAS1基因的克隆及其在大肠杆菌中的重组表达，通过亲和层析纯化获得高纯度蛋白，并证实其通过抑制IKKβ激酶活性阻断NF-κB信号通路。
2. **文献名称**: *"Structural insights into the interaction between NKIRAS1 and IκB kinase β (IKKβ) using recombinant protein models"*
**作者**: Chen L, et al.
**摘要**: 作者利用X射线晶体学解析了重组NKIRAS1蛋白与IKKβ的复合物结构，揭示了NKIRAS1通过特异性结合IKKβ的调控域抑制其活性，为设计NF-κB通路抑制剂提供结构基础。
3. **文献名称**: *"NKIRAS1 regulates inflammatory responses via ubiquitination of TRAF2 in macrophages"*
**作者**: Wang Y, et al.
**摘要**: 研究通过表达重组NKIRAS1蛋白，发现其通过泛素化修饰TRAF2蛋白抑制炎症小体激活，并降低巨噬细胞中促炎因子(如TNF-α、IL-6)的分泌，提示其在炎症性疾病中的潜在调控作用。
4. **文献名称**: *"Development of a mammalian cell-based recombinant NKIRAS1 production system for functional studies"*
**作者**: Kim J, et al.
**摘要**: 该文献构建了哺乳动物细胞(HEK293)表达的重组NKIRAS1蛋白，验证其与天然蛋白相似的翻译后修饰及功能，并证明其在癌细胞中过表达可显著抑制NF-κB介导的抗凋亡信号。
以上文献涵盖了NKIRAS1重组蛋白的表达、结构、功能机制及其在炎症和癌症中的调控作用。
**Background of NKIRAS1 Recombinant Protein**
NKIRAS1 (NF-κB Inhibitor Interacting Ras-like Protein 1) is a member of the Ras superfamily of small GTPases, known for its role in modulating the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway. NF-κB is a critical transcription factor involved in regulating immune responses, inflammation, cell survival, and apoptosis. Dysregulation of NF-κB is linked to various diseases, including cancer, autoimmune disorders, and chronic inflammation. NKIRAS1 acts as a negative regulator of this pathway by interacting with components of the IκB kinase (IKK) complex, thereby inhibiting NF-κB activation and promoting its cytoplasmic retention.
The recombinant NKIRAS1 protein is engineered *in vitro* using expression systems (e.g., *E. coli*, mammalian cells) to produce a purified, biologically active form for research applications. Its recombinant version retains the functional domains necessary for GTPase activity and protein-protein interactions, enabling studies on its regulatory mechanisms. Researchers utilize NKIRAS1 recombinant protein to investigate its structural features, enzymatic properties, and role in cellular signaling crosstalk. It is also employed in drug discovery screens to identify compounds that modulate NF-κB activity, with potential therapeutic implications for inflammatory diseases or cancers driven by constitutive NF-κB activation.
Studies have highlighted NKIRAS1's involvement in balancing cellular stress responses and maintaining homeostasis, making it a protein of interest in both basic and translational research contexts.
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