| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHP2L1 |
| Uniprot No | P55769 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMTEADVNPKAYPLADAHLTKKLLDLV QQSCNYKQLRKGANEATKTLNRGISEFIVMAADAEPLEIILHLPLLCEDK NVPYVFVRSKQALGRACGVSRPVIACSVTIKEGSQLKQQIQSIQQSIERL LV |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NHP2L1重组蛋白的3篇模拟参考文献，格式符合要求：
1. **文献名称**：*Structural and functional analysis of recombinant NHP2L1 in spliceosome assembly*
**作者**：Tanaka, R. et al.
**摘要**：通过大肠杆菌表达系统纯化重组NHP2L1蛋白，结合冷冻电镜技术解析其与U4 snRNA的相互作用位点，揭示其在剪接体早期组装中的关键构象变化。
2. **文献名称**：*Recombinant NHP2L1 binds to the 15.5K protein-RNA complex with high specificity*
**作者**：Vidal, S. et al.
**摘要**：利用体外结合实验证明重组NHP2L1与15.5K蛋白及U4 snRNA形成稳定复合物，通过突变分析确定其RNA结合域对剪接体功能的必要性。
3. **文献名称**：*Dysregulation of NHP2L1 expression impairs ribosome biogenesis via recombinant protein interaction assays*
**作者**：Wang, L. et al.
**摘要**：研究重组NHP2L1在核糖体RNA加工中的作用，发现其通过与核仁蛋白NOP56的相互作用调控rRNA前体的甲基化修饰。
(注：以上文献为模拟内容，实际引用时需核实真实来源。)
NHP2L1 (SNU13 homolog, H/ACA ribonucleoprotein subunit) is a highly conserved eukaryotic protein integral to RNA metabolism and ribosome biogenesis. As a core component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex, it plays a critical role in guiding site-specific pseudouridylation of ribosomal RNA (rRNA), a post-transcriptional modification essential for ribosome assembly and translational fidelity. Structurally, NHP2L1 contains a canonical NHP2-like domain that mediates interactions with other snoRNP proteins, including dyskerin, GAR1. and NOP10. forming a scaffold for small nucleolar RNA (snoRNA) binding. Its function extends beyond rRNA modification, with involvement in telomerase RNA maturation and spliceosomal snRNA processing.
Recombinant NHP2L1 protein is engineered for in vitro studies to dissect molecular mechanisms underlying snoRNP assembly, RNA-protein interactions, and pseudouridylation catalysis. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains native folding and biochemical activity, enabling structural analyses (e.g., X-ray crystallography) and functional assays. Researchers utilize recombinant NHP2L1 to investigate mutations linked to human diseases, such as congenital dyskeratosis and certain cancers, where snoRNP dysfunction disrupts ribosome homeostasis or telomere maintenance. Additionally, it serves as a tool for screening therapeutic compounds targeting RNA-protein networks. The protein’s conserved nature across species further supports cross-disciplinary studies in genetics, molecular biology, and translational medicine.
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