| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NIP7 |
| Uniprot No | Q9Y221 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEK IMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIK PGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKST QDCRKVDPMAIVVFHQADIGEYVRHEETLT |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NIP7重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供格式参考)：
1. **文献名称**: *Functional characterization of NIP7 in ribosomal RNA processing*
**作者**: Smith J, et al.
**摘要**: 研究利用重组NIP7蛋白分析其在真核生物核糖体RNA前体加工中的作用，揭示其通过结合特定RNA结构域促进rRNA成熟及核糖体亚基组装。
2. **文献名称**: *Expression and purification of recombinant NIP7 for structural studies*
**作者**: Lee S, et al.
**摘要**: 报道了一种在大肠杆菌中高效表达并纯化重组NIP7蛋白的方法，通过X射线晶体学解析其三维结构，阐明其RNA结合位点的分子机制。
3. **文献名称**: *NIP7 deficiency disrupts ribosome biogenesis and links to human developmental disorders*
**作者**: Garcia R, et al.
**摘要**: 利用重组NIP7蛋白进行功能拯救实验，证明NIP7缺失导致细胞核仁结构异常和rRNA加工停滞，与人类神经发育障碍相关。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“NIP7 recombinant protein”或“NIP7 ribosome biogenesis”。
NIP7 (Nuclear Integral Protein 7) is a conserved eukaryotic protein implicated in ribosome biogenesis and RNA processing. Initially identified in yeast as a nucleolar component, NIP7 plays a critical role in the maturation of ribosomal RNA (rRNA), particularly during the cleavage and modification stages of 18S rRNA within the small ribosomal subunit. Structurally, NIP7 contains a RNA-binding domain and interacts with other ribosome assembly factors, such as Nop8 and Rrp14. forming complexes essential for pre-rRNA processing. Studies in humans and model organisms suggest its involvement in maintaining nucleolar architecture and coordinating cell cycle progression. Dysregulation of NIP7 has been linked to developmental defects and diseases, including ribosomopathies and certain cancers, highlighting its biological significance.
Recombinant NIP7 protein is typically produced using heterologous expression systems (e.g., *E. coli* or yeast) to enable functional and structural studies. The recombinant form allows researchers to investigate its molecular interactions, enzymatic roles, and structural contributions to ribosome assembly *in vitro*. Purification often involves affinity tags (e.g., His-tag) followed by chromatography techniques. Its applications span structural biology (e.g., X-ray crystallography, cryo-EM), RNA-protein interaction assays, and drug discovery targeting ribosome-related disorders. Recent studies also explore its potential as a biomarker for cellular stress responses. The development of recombinant NIP7 has significantly advanced our understanding of ribosome biogenesis mechanisms and their implications in human health.
×
