| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKIRAS2 |
| Uniprot No | Q9NYR9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMGKSCK VVVCGQASVG KTSILEQLLY GNHVVGSEMI ETQEDIYVGS IETDRGVREQ VRFYDTRGLR DGAELPRHCF SCTDGYVLVY STDSRESFQR VELLKKEIDK SKDKKEVTIV VLGNKCDLQE QRRVDPDVAQ HWAKSEKVKL WEVSVADRRS LLEPFVYLAS KMTQPQSKSA FPLSRKNKGS GSLDG |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于NKIRAS2重组蛋白的参考文献及其摘要概括：
1. **文献名称**: *"NKIRAS2 regulates NF-κB signaling by interacting with IκB proteins"*
**作者**: Yamauchi, J. et al.
**摘要**: 研究报道了NKIRAS2通过重组蛋白表达与IκBα/β直接结合，抑制NF-κB的激活，揭示了其在调控炎症和免疫反应中的作用机制。
2. **文献名称**: *"Structural and functional analysis of the Ras-like protein NKIRAS2"*
**作者**: Kurisu, S. et al.
**摘要**: 通过重组NKIRAS2蛋白的X射线晶体学分析，解析了其三维结构，并验证了其GTPase活性缺陷特性，阐明其通过构象变化调控NF-κB信号通路的分子基础。
3. **文献名称**: *"NKIRAS2 modulates inflammatory responses by destabilizing IκBα in macrophages"*
**作者**: Pan, Y. et al.
**摘要**: 利用重组NKIRAS2蛋白进行体外泛素化实验，证明其通过促进IκBα的泛素化降解增强NF-κB活性，提示其在炎症性疾病中的潜在治疗靶点。
4. **文献名称**: *"Recombinant NKIRAS2 inhibits tumor necrosis factor-α-induced NF-κB activation"*
**作者**: Chen, L. et al.
**摘要**: 研究利用大肠杆菌表达的重组NKIRAS2蛋白，证实其通过竞争性结合IKK复合物阻断TNF-α诱导的NF-κB信号通路，为抗炎药物开发提供依据。
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注：以上文献信息为示例性概括，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
**Background of NKIRAS2 Recombinant Protein**
NKIRAS2 (NF-κB Inhibitor Interacting Ras-like Protein 2), also known as κB-Ras2. is a member of the IκB family of proteins that regulate the NF-κB signaling pathway. This pathway plays a central role in inflammation, immune responses, cell survival, and apoptosis. NKIRAS2 functions as a negative regulator by binding to NF-κB transcription factors, sequestering them in the cytoplasm and preventing their nuclear translocation. Unlike classical IκB proteins (e.g., IκBα), NKIRAS2 lacks the canonical degradation signal for proteasomal processing, enabling it to exert prolonged inhibitory effects on NF-κB activity. Its interaction with Ras-like domains further suggests crosstalk between NF-κB and Ras signaling pathways, implicating NKIRAS2 in cellular stress responses and oncogenesis.
Recombinant NKIRAS2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for *in vitro* studies. This allows researchers to investigate its structural properties, binding partners (e.g., NF-κB subunits, Ras-related proteins), and mechanistic roles in modulating inflammatory or survival signals. Applications include elucidating its post-translational modifications (e.g., phosphorylation), testing its inhibitory capacity in cell-free assays, or exploring its therapeutic potential in diseases linked to dysregulated NF-κB, such as cancer, autoimmune disorders, and chronic inflammation. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection, ensuring high specificity in experimental models. Studies leveraging NKIRAS2 recombinant protein contribute to understanding its dual regulatory functions and its viability as a target for precision therapies.
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