Recombinant Human NKIRAS2 protein

**Background of NKIRAS2 Recombinant Protein**

NKIRAS2 (NF-κB Inhibitor Interacting Ras-like Protein 2), also known as κB-Ras2. is a member of the IκB family of proteins that regulate the NF-κB signaling pathway. This pathway plays a central role in inflammation, immune responses, cell survival, and apoptosis. NKIRAS2 functions as a negative regulator by binding to NF-κB transcription factors, sequestering them in the cytoplasm and preventing their nuclear translocation. Unlike classical IκB proteins (e.g., IκBα), NKIRAS2 lacks the canonical degradation signal for proteasomal processing, enabling it to exert prolonged inhibitory effects on NF-κB activity. Its interaction with Ras-like domains further suggests crosstalk between NF-κB and Ras signaling pathways, implicating NKIRAS2 in cellular stress responses and oncogenesis.

Recombinant NKIRAS2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for *in vitro* studies. This allows researchers to investigate its structural properties, binding partners (e.g., NF-κB subunits, Ras-related proteins), and mechanistic roles in modulating inflammatory or survival signals. Applications include elucidating its post-translational modifications (e.g., phosphorylation), testing its inhibitory capacity in cell-free assays, or exploring its therapeutic potential in diseases linked to dysregulated NF-κB, such as cancer, autoimmune disorders, and chronic inflammation. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection, ensuring high specificity in experimental models. Studies leveraging NKIRAS2 recombinant protein contribute to understanding its dual regulatory functions and its viability as a target for precision therapies.