| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nkx3-1 |
| Uniprot No | Q99801 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMLRVPEPRPGEAKAEGAAPPTPSKPLT SFLIQDILRDGAQRQGGRTSSQRQRDPEPEPEPEPEGGRSRAGAQNDQLS TGPRAAPEEAETLAETEPERHLGSYLLDSENTSGALPRLPQTPKQPQKRS RAAFSHTQVIELERKFSHQKYLSAPERAHLAKNLKLTETQVKIWFQNRRY KTKRKQLSSELGDLEKHSSLPALKEEAFSRASLVSVYNSYPYYPYLYCVG SWSPAFW |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nkx3-1重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*NKX3.1 stabilizes p53. inhibits AKT activation, and blocks prostate cancer initiation*
**作者**：Abdullah, C. et al.
**摘要**：该研究利用重组NKX3.1蛋白进行体外实验，发现其通过与p53直接互作增强肿瘤抑制功能，并抑制PI3K/AKT通路，从而延缓前列腺癌进展。
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2. **文献名称**：*DNA recognition by the Nkx3.1 homeodomain involves an induced fit mechanism*
**作者**：Kim, S. et al.
**摘要**：通过X射线晶体学解析重组NKX3.1蛋白的DNA结合结构域，揭示其通过构象变化特异性识别靶DNA序列，为前列腺发育相关基因调控提供结构基础。
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3. **文献名称**：*Prostate-specific expression of the homeobox gene NKX3.1*
**作者**：Bieberich, C.J. et al.
**摘要**：首次报道重组NKX3.1蛋白在前列腺组织中的特异性表达模式，并验证其通过调控PSA等基因维持前列腺上皮细胞分化，提示其在器官发育中的关键作用。
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4. **文献名称**：*Purification and functional analysis of recombinant NKX3.1 for chromatin interaction studies*
**作者**：Steadman, D.J. et al.
**摘要**：开发了一种高效的大肠杆菌表达系统纯化重组NKX3.1蛋白，结合ChIP-seq技术揭示其在前列腺细胞中靶向结合的染色质区域及表观遗传调控机制。
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注：上述文献为模拟示例，实际引用时需核对真实出版物信息。如需具体文献，建议通过PubMed或Google Scholar检索关键词“NKX3-1 recombinant protein”。
**Background of Nkx3-1 Recombinant Protein**
Nkx3-1 (NK3 homeobox 1) is a transcription factor belonging to the NKX family of homeodomain-containing proteins, which play critical roles in developmental processes and tissue homeostasis. It is encoded by the *NKX3-1* gene, a conserved regulator predominantly expressed in mammalian prostate, testis, bladder, and regions of the central nervous system. Nkx3-1 functions as a tumor suppressor, particularly in prostate development and carcinogenesis, where it regulates epithelial cell differentiation, proliferation, and apoptosis. Its loss or reduced expression is closely associated with prostate intraepithelial neoplasia and adenocarcinoma.
The recombinant Nkx3-1 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cell lines) to produce a purified, functional form of the protein. This involves inserting the *NKX3-1* cDNA into an expression vector, followed by transfection, protein synthesis, and affinity chromatography-based purification. The recombinant protein retains key functional domains, including the N-terminal transcriptional repression domain and the C-terminal homeodomain responsible for DNA binding.
Researchers utilize recombinant Nkx3-1 to study its molecular mechanisms, such as DNA-protein interactions, co-regulator partnerships, and downstream target gene regulation (e.g., *PSA*, *AR*). It is also employed in disease modeling, drug screening, and exploring pathways linked to prostate cancer progression or genitourinary developmental disorders. Additionally, post-translational modifications (e.g., phosphorylation) impacting its stability and activity are often analyzed using recombinant variants.
Overall, Nkx3-1 recombinant protein serves as a vital tool for dissecting its tumor-suppressive roles and developing therapeutic strategies targeting pathways influenced by its dysfunction.
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