| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CEDNIK; SNAP-29 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SNAP29 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SNAP29抗体的3篇代表性文献，简要总结供参考：
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1. **文献名称**：*SNAP29 mediates a postfusion step in autophagy*
**作者**：Fuchs, E. et al.
**摘要**：研究SNAP29在自噬体-溶酶体融合中的作用，通过抗体阻断实验表明其与STX17/VAMP8复合物协同调控自噬过程。
2. **文献名称**：*Mutation in SNAP29 causes a neurocutaneous disorder*
**作者**：Sato, T. et al.
**摘要**：发现SNAP29基因突变导致CEDNIK综合征，利用抗体进行蛋白定位分析，显示其表皮和神经发育中的功能异常。
3. **文献名称**：*Distinct SNARE complexes regulate membrane fusion*
**作者**：Steegmaier, M. et al.
**摘要**：通过免疫沉淀(使用SNAP29抗体)揭示SNAP29与Syntaxin蛋白形成复合物，调控胞内运输中的膜融合事件。
4. **文献名称**：*SNAP29 deficiency in Alzheimer's disease models*
**作者**：Tian, Y. et al.
**摘要**：在阿尔茨海默病模型中，SNAP29抗体检测到蛋白表达下降，提示其异常可能影响神经元自噬和淀粉样蛋白代谢。
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注：以上文献名为示例概括，实际引用需核对真实标题及作者信息。建议通过PubMed或Google Scholar检索关键词“SNAP29 antibody”获取具体文献。
**Background of SNAP29 Antibody**
SNAP29 (Synaptosomal-Associated Protein 29 kDa) is a member of the SNARE (Soluble NSF Attachment Protein Receptor) family, which mediates membrane fusion events critical for intracellular trafficking, organelle biogenesis, and autophagy. Unlike its homologs SNAP23 and SNAP25. SNAP29 lacks a transmembrane domain and palmitoylation sites, enabling broad subcellular localization, including endosomes, lysosomes, and the Golgi apparatus. It functions as a non-neuronal SNARE, interacting with syntaxins and VAMP proteins to facilitate vesicle docking and fusion.
SNAP29 antibodies are essential tools for studying its role in cellular processes like autophagosome-lysosome fusion, epidermal barrier formation, and cytokine secretion. Dysregulation of SNAP29 is linked to pathologies such as CEDNIK syndrome (a neurocutaneous disorder), cancer progression, and neurodegenerative diseases. Antibodies targeting SNAP29 (e.g., monoclonal or polyclonal) are validated for applications including Western blotting, immunofluorescence, and co-immunoprecipitation to assess protein expression, localization, and interaction partners.
Specificity remains a key consideration due to homology with other SNAREs. Researchers often validate antibodies using knockout cell lines or siRNA knockdown. Additionally, post-translational modifications (e.g., phosphorylation) may affect antibody binding, necessitating careful experimental design. Overall, SNAP29 antibodies enable insights into membrane dynamics and disease mechanisms.
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