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Rabbit Polyclonal SMYD3 Antibody

  • 中文名: SMYD3抗体
  • 别    名: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
货号: IPDX08785
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/500-1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesKMT3E; ZMYND1; ZNFN3A1; bA74P14.1
WB Predicted band size49 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human SMYD3
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3篇关于SMYD3抗体的虚构参考文献示例(内容基于领域知识模拟,非真实文献):

1. **文献名称**:*SMYD3-specific monoclonal antibody development and its application in cancer biomarker detection*

**作者**:Hamamoto R, et al.

**摘要**:本研究开发了一种高特异性SMYD3单克隆抗体,并通过Western blot和免疫组化验证其在多种癌症细胞系及组织中的表达,证实SMYD3在结直肠癌中异常高表达并与患者预后相关。

2. **文献名称**:*Epigenetic regulation by SMYD3: Validation of a polyclonal antibody for chromatin immunoprecipitation*

**作者**:Sarris ME, et al.

**摘要**:文章报道了一种经ChIP-seq验证的SMYD3多克隆抗体,证明其能特异性识别SMYD3结合的染色质区域,并揭示SMYD3通过H3K4甲基化调控致癌基因转录的分子机制。

3. **文献名称**:*Comparative analysis of commercial SMYD3 antibodies for functional studies*

**作者**:Zhu H, et al.

**摘要**:系统评估了5种市售SMYD3抗体的特异性及适用性,发现抗体Clone 2B7在免疫荧光和流式细胞术中表现最优,为SMYD3功能研究提供了实验依据。

4. **文献名称**:*SMYD3 knockout mouse model reveals antibody-based detection of protein localization in tumor microenvironment*

**作者**:Tsuge M, et al.

**摘要**:利用SMYD3基因敲除小鼠模型,验证了一种兔源SMYD3抗体的特异性,并证明SMYD3在肿瘤相关成纤维细胞中的定位与癌症转移相关。

(注:以上文献为模拟生成,实际研究中请通过PubMed或Google Scholar查询真实文献。)

背景信息

SMYD3 (SET and MYND domain-containing protein 3) is a lysine methyltransferase that plays a critical role in epigenetic regulation by catalyzing the methylation of histone H3 lysine 4 (H3K4) and non-histone targets. It has been implicated in transcriptional activation, cell cycle progression, and oncogenesis. Overexpression of SMYD3 is associated with various cancers, including liver, breast, pancreatic, and colorectal cancers, where it promotes tumor proliferation, metastasis, and drug resistance by modulating cancer-related pathways such as Wnt/β-catenin and MAPK/ERK. SMYD3 antibodies are essential tools for detecting and quantifying SMYD3 expression in research and diagnostic contexts. These antibodies, often generated in rabbits or mice using immunogenic peptide sequences, are validated for specificity through techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They enable the study of SMYD3's subcellular localization, interaction partners, and dysregulation in diseases. Recent studies also explore SMYD3 as a potential therapeutic target, with antibodies aiding in preclinical evaluations of inhibitors. Reliable SMYD3 antibodies are crucial for advancing cancer biology research and developing targeted epigenetic therapies.

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