| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/400 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SVAP1; HEL170; SNAP-47; C1orf142; ESFI5812; HEL-S-290 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human SNAP47 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SNAP47抗体的3篇参考文献概述：
1. **"SNAP47 regulates cargo delivery via selective association with a subset of vesicle-associated membrane proteins"**
*作者：Zhang et al. (2018)*
摘要：研究揭示了SNAP47通过与特定VAMP蛋白(如VAMP7)的相互作用调控内体-溶酶体系统的膜融合过程，使用特异性抗体验证其在细胞内的定位及功能。
2. **"Distinct SNARE complexes mediating membrane fusion in different cellular compartments"**
*作者：Wang & Tang (2016)*
摘要：通过免疫沉淀和Western blot分析，发现SNAP47与Syntaxin6/VAMP4复合物结合，参与高尔基体到内体的运输，抗体实验证实其在不同细胞器中的分布差异。
3. **"SNAP47 interacts with ATG14 to modulate autophagosome-lysosome fusion"**
*作者：Chen et al. (2020)*
摘要：研究利用SNAP47抗体进行共定位及敲低实验，证明SNAP47通过与自噬相关蛋白ATG14结合，影响自噬体与溶酶体的融合效率。
The SNAP47 antibody is a research tool designed to detect SNAP47 (Synaptosomal-associated protein 47), a member of the SNARE (soluble NSF attachment protein receptor) family involved in intracellular membrane fusion processes. SNAP47. encoded by the SNAP47 gene in humans, shares structural homology with other SNAP proteins like SNAP25 and SNAP29 but exhibits distinct localization and functional roles. It is broadly expressed across tissues and participates in diverse trafficking pathways, including endosomal sorting, autophagosome-lysosome fusion, and unconventional secretion. Unlike its homologs, SNAP47 localizes to both neuronal and non-neuronal compartments, suggesting versatility in membrane dynamics.
Researchers utilize SNAP47 antibodies primarily in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its expression, interaction partners, and subcellular distribution. These antibodies are critical for exploring SNAP47’s role in cellular homeostasis, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where disrupted membrane trafficking is implicated. Commercial SNAP47 antibodies are often validated for specificity against recombinant proteins or knockout cell lines to minimize cross-reactivity with other SNAREs. Challenges include distinguishing isoforms and detecting low-abundance endogenous SNAP47 in certain cell types. Recent studies highlight its involvement in LC3-associated phagocytosis and mTOR signaling, underscoring its emerging importance in cell biology and therapeutic targeting.
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