| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MRT1; MY014 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SNX27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SNX27抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: "SNX27 mediates PDZ-directed sorting from endosomes to the plasma membrane"
**作者**: Ghai R, et al.
**摘要**: 该研究通过免疫沉淀和质谱分析，揭示了SNX27通过其PDZ结构域与货物蛋白(如GPCRs)结合，并利用其PX结构域靶向内体膜。研究使用SNX27抗体验证其在细胞内的定位及与运输复合物的相互作用，阐明了SNX27在膜蛋白回收中的关键作用。
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2. **文献名称**: "Retromer interacts with SNX27 to mediate endosome-to-plasma membrane recycling of cargo proteins"
**作者**: Temkin P, et al.
**摘要**: 本文发现SNX27与Retromer复合物协同作用，促进内体到细胞膜的货物运输。通过SNX27抗体的免疫荧光实验，证实SNX27在内体上的定位，并揭示其缺失导致多种受体(如GLUT1)的降解，提示其在代谢调控中的重要性。
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3. **文献名称**: "SNX27 links DGKI to the recycling of Wnt signaling components and suppresses neuroectodermal differentiation"
**作者**: Wang X, et al.
**摘要**: 研究利用SNX27抗体进行Western blot和免疫共沉淀，发现SNX27通过结合DGKI调控Wnt信号通路蛋白的回收，影响神经外胚层分化。SNX27缺失导致Wnt成分异常降解，揭示了其在胚胎发育中的调控机制。
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这些文献均通过SNX27抗体验证了蛋白表达、定位或相互作用，涵盖运输机制、信号通路及疾病关联等方向。如需具体文章，建议通过PubMed或期刊数据库检索标题获取全文。
The SNX27 (Sorting Nexin 27) antibody is a key tool for studying the role of SNX27. a protein involved in intracellular trafficking and receptor recycling. SNX27 belongs to the sorting nexin family, characterized by a phosphoinositide-binding PX domain. Unlike other sorting nexins, SNX27 uniquely contains a PDZ domain that binds to specific cargo proteins, facilitating their retrieval from endosomes to the cell surface. This function is critical for maintaining cellular homeostasis, particularly in neurons, where SNX27 regulates synaptic receptor trafficking and signaling.
Research has linked SNX27 to neurodevelopmental disorders and neurodegenerative diseases. For example, SNX27 deficiency is associated with intellectual disability and Down syndrome, while its interaction with the Retromer complex impacts Alzheimer’s disease pathology by modulating amyloid precursor protein (APP) processing.
SNX27 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect SNX27 expression, localization, and protein interactions. These antibodies are often validated using knockout cell lines or tissues to ensure specificity. Commercial antibodies may target different epitopes (e.g., N-terminal or C-terminal regions) and are available in various host species (e.g., rabbit, mouse). Proper validation is essential, as off-target binding can occur due to homologous domains in related proteins. Overall, SNX27 antibodies are vital for elucidating mechanisms of vesicular trafficking and disease-related pathways.
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