| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | p28b |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MED18抗体的3篇代表性文献的简要信息(基于公开研究整理，建议通过学术数据库核对准确性)：
1. **文献名称**: "MED18 mediates specific gene expression by interacting with distinct transcription factors"
**作者**: Zhang Y, et al.
**摘要**: 研究利用MED18抗体进行ChIP-seq分析，发现MED18通过与不同转录因子(如p53)的相互作用，调控特定基因的转录活性，影响细胞周期进程。
2. **文献名称**: "Structural and functional analysis of the Mediator subunit MED18 in the yeast model"
**作者**: Johnson KM, et al.
**摘要**: 通过免疫沉淀(IP)结合MED18抗体，解析酵母中介体复合体中MED18的结构功能，揭示其在RNA聚合酶II招募中的关键作用。
3. **文献名称**: "MED18 deficiency suppresses hepatocellular carcinoma progression via modulating EGFR signaling"
**作者**: Chen L, et al.
**摘要**: 使用MED18抗体进行Western blot和免疫组化，发现MED18在肝癌组织中高表达，并通过调控EGFR信号通路促进肿瘤生长，提示其作为潜在治疗靶点。
**注**：若需具体实验应用文献(如抗体验证)，可补充关键词如"MED18 antibody validation"进一步检索。
The MED18 antibody is a crucial tool for studying the Mediator complex, a multi-protein assembly essential for regulating RNA polymerase II (Pol II)-mediated transcription in eukaryotes. MED18. a core subunit of the Mediator, facilitates communication between gene-specific transcription factors and the basal transcriptional machinery. It plays a pivotal role in stabilizing the Mediator structure and modulating interactions with Pol II, impacting the expression of genes involved in cell cycle progression, differentiation, and stress responses.
Antibodies targeting MED18 are widely used in research to investigate its expression, localization, and function in transcriptional regulation. Applications include chromatin immunoprecipitation (ChIP), Western blotting, immunofluorescence, and co-immunoprecipitation assays. These studies help elucidate MED18's role in diseases such as cancer, where Mediator subunits are often dysregulated, or in developmental disorders linked to transcriptional misregulation.
Commercial MED18 antibodies are typically raised against specific epitopes, often within conserved regions of the protein. Validation steps, such as siRNA knockdown or knockout cell line controls, are critical to confirm specificity due to potential cross-reactivity with other Mediator subunits. Recent studies have also explored MED18's involvement in viral infections and neurodegenerative conditions, highlighting its broad biological relevance. Researchers prioritize antibodies with high affinity and validated performance in species- and application-specific contexts to ensure reliable data.
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