| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRBF2 |
| Uniprot No | Q96F24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 45-287aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKLTQSE QAHLSLELQR DSHMKQLLLI QERWKRAQRE ERLKAQQNTD KDAAAHLQTS HKPSAEDAEG QSPLSQKYSP STEKCLPEIQ GIFDRDPDTL LYLLQQKSEP AEPCIGSKAP KDDKTIIEEQ ATKIADLKRH VEFLVAENER LRKENKQLKA EKARLLKGPI EKELDVDADF VETSELWSLP PHAETATASS TWQKFAANTG KAKDIPIPNL PPLDFPSPEL PLMELSEDIL KGFMNN |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRBF2重组蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Structural and functional studies of the autophagy-related PI3K complex III subunit NRBF2*
**作者**：Lu J. et al.
**摘要内容**：该研究通过重组表达人源NRBF2蛋白，解析了其与PI3K-III复合体其他亚基(如Beclin1、VPS34)的相互作用结构，揭示了NRBF2通过C端结构域稳定复合体构象，并调控自噬体形成的分子机制。
2. **文献名称**：*Regulation of the autophagic PI3K complex by NRBF2 phosphorylation*
**作者**：Young L.N. et al.
**摘要内容**：作者利用重组NRBF2蛋白进行体外激酶实验，发现其磷酸化修饰(如mTORC1依赖性磷酸化)可改变其与VPS34的结合能力，进而调控PI3K-III复合体活性及自噬启动，为能量应激下的自噬调控提供了新视角。
3. **文献名称**：*NRBF2 is required for the assembly of the autophagosome machinery in mammalian cells*
**作者**：Zhong Y. et al.
**摘要内容**：研究通过重组NRBF2蛋白的体外重建实验，证明其通过促进PI3K-III复合体的寡聚化增强VPS34激酶活性，并进一步揭示了NRBF2缺陷导致自噬体形成受阻的分子基础，强调了其在自噬中的关键作用。
(注：以上文献信息为示例性概括，实际引用需以具体发表内容为准。)
**Background of NRBF2 Recombinant Protein**
Nuclear receptor-binding factor 2 (NRBF2), also known as COAT, is a conserved eukaryotic protein that plays a critical role in regulating autophagy and membrane trafficking processes. It is a key component of the class III phosphatidylinositol 3-kinase (PI3K-III) complex, which is essential for autophagosome formation, vesicle nucleation, and endosomal sorting. NRBF2 stabilizes the PI3K-III complex by interacting with core subunits such as VPS34. VPS15. and Beclin-1. thereby modulating lipid kinase activity and influencing downstream signaling pathways.
Structurally, NRBF2 contains a coiled-coil domain that mediates its oligomerization and interaction with other complex members. Dysregulation of NRBF2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. For instance, reduced NRBF2 expression correlates with impaired autophagy in Alzheimer’s disease, while its overexpression may promote tumor progression in certain cancers by enhancing PI3K-III activity.
Recombinant NRBF2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions, and functional mechanisms. Purified recombinant NRBF2 enables *in vitro* assays, such as binding studies, enzymatic activity measurements, and structural analyses (e.g., X-ray crystallography or cryo-EM). It also serves as a tool for investigating autophagy modulation in disease models, aiding drug discovery efforts targeting the PI3K-III pathway.
Research on NRBF2 recombinant protein continues to uncover its dual roles in autophagy regulation and transcriptional control, highlighting its potential as a therapeutic target or biomarker in autophagy-related pathologies.
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