| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPM2 |
| Uniprot No | Q86SE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214aa |
| 氨基酸序列 | MNLSSASSTE EKAVTTVLWG CELSQERRTW TFRPQLEGKQ SCRLLLHTIC LGEKAKEEMH RVEILPPANQ EDKKMQPVTI ASLQASVLPM VSMVGVQLSP PVTFQLRAGS GPVFLSGQER YEASDLTWEE EEEEEGEEEE EEEEDDEDED ADISLEEQSP VKQVKRLVPQ KQASVAKKKK LEKEEEEIRA SVRDKSPVKK AKATARAKKP GFKK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPM2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "Nucleoplasmin 2 is required for chromosomal compactions and early embryo development"
**作者**: Inoue A, et al.
**摘要**: 本研究通过重组NPM2蛋白的体外实验，揭示了其在胚胎发育早期对染色质结构和染色体凝缩的关键作用。实验表明，重组NPM2通过结合组蛋白H2A-H2B并促进其从染色质中解离，从而调控染色质动态重组。
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2. **文献名称**: "Recombinant NPM2 binds to pericentromeric heterochromatin and regulates genome stability"
**作者**: Frehlick LJ, et al.
**摘要**: 该文献利用重组NPM2蛋白分析其与异染色质的相互作用，发现NPM2通过结合特定卫星DNA序列维持基因组稳定性。研究还表明，重组NPM2的缺失会导致卵母细胞中异染色质结构的异常和染色体分离错误。
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3. **文献名称**: "Functional characterization of NPM2 in oocyte maturation using recombinant protein-based assays"
**作者**: Zhang Y, et al.
**摘要**: 通过体外表达和纯化重组NPM2蛋白，本研究揭示了其在卵母细胞成熟过程中对核仁组装和核质运输的调控机制。实验证明，NPM2重组蛋白能够恢复NPM2缺陷卵母细胞的发育停滞表型。
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如果需要更具体的文献或补充信息，可进一步说明研究方向(如结构、疾病关联等)。
**Background of NPM2 Recombinant Protein**
NPM2 (Nucleophosmin 2), a member of the nucleophosmin/nucleoplasmin (NPM) family, is a nuclear chaperone protein critical for chromatin organization and early embryonic development. Unlike its well-studied paralog NPM1. which is involved in ribosome biogenesis and tumorigenesis, NPM2 is primarily associated with reproductive biology. It plays a key role in remodeling sperm chromatin after fertilization by displacing protamines with histones, facilitating proper zygotic genome activation. Studies in knockout models highlight its necessity for embryonic viability, as NPM2-deficient mice exhibit developmental arrest at the 2-cell stage.
Recombinant NPM2 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures*) to enable functional studies. Its recombinant form retains the ability to bind histones and modulate chromatin structure, making it a valuable tool for investigating epigenetic regulation, nucleosome assembly, and early embryogenesis *in vitro*. Research also explores its potential role in cancer, as aberrant NPM2 expression has been linked to genomic instability and tumor progression.
The development of NPM2 recombinant protein addresses challenges in studying native NPM2. which is low in abundance and difficult to isolate. Its availability supports advances in reproductive medicine, developmental biology, and oncology, offering insights into molecular mechanisms of chromatin dynamics and therapeutic targeting.
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