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Mouse Glucagon-like peptide 1 receptor(GLP1R) ELISA kit

  • 中文名: 小鼠胰高血糖素样肽1受体(GLP1R)酶联免疫试剂盒
货号: IPD-EL009514MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠胰高血糖素样肽1受体(GLP1R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GLP1R含量。GLP1R即胰高血糖素样肽 -1 受体,在调节血糖、控制体重等方面有重要作用。其研究机制主要是当 GLP1 与 GLP1R 结合后,可刺激胰岛素分泌、抑制胰高血糖素释放、延缓胃排空,目前基于此靶点开发的药物在糖尿病和肥胖症治疗领域应用广泛。试剂盒检测范围为0.45 ng/ml- 30 ng/ml,灵敏度为0.11 ng/ml。适用于科研场景中评估小鼠模型在疾病状态或药物干预下GLP1R的表达动态,例如探究GLP - 1类似物对受体激活的影响、代谢综合征病理机制分析或肠道 - 脑轴信号传导研究。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Glp1rGlucagon-like peptide 1 receptor ELISA kit; GLP-1 receptor ELISA kit; GLP-1-R ELISA kit; GLP-1R ELISA kit
  • 缩写:
    GLP1R
  • Uniprot No.:
    O35659
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.45 ng/ml - 30 ng/ml
  • 灵敏度:
    0.11 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    90-99


    1:2

    Average %

    89


    Range %

    84-93


    1:4

    Average %

    92


    Range %

    88-96


    1:8

    Average %

    104


    Range %

    100-110


  • 回收率:

    The recovery of mouse GLP1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    93-105


    EDTA plasma (n=4)

    93

    89-98


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    30

    2.692

    2.627

    2.660

    2.542


    15

    2.107

    2.256

    2.182

    2.064


    7.5

    1.573

    1.651

    1.612

    1.494


    3.75

    1.002

    0.948

    0.975

    0.857


    1.8

    0.560

    0.549

    0.555

    0.437


    0.9

    0.354

    0.331

    0.343

    0.225


    0.45

    0.237

    0.254

    0.246

    0.128


    0

    0.121

    0.115

    0.118




  • 货期:
    3-5 working days

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