| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ROS; MCF3; c-ros-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ROS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNAJB11抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*DNAJB11 mutations cause autosomal dominant polycystic kidney disease by disrupting HSP40 co-chaperone function*
**作者**：Cornec-Le Gall E, et al.
**摘要**：该研究通过全外显子测序发现DNAJB11基因突变与常染色体显性多囊肾病(ADPKD)相关，并利用DNAJB11抗体进行蛋白质表达分析，揭示了DNAJB11作为HSP40辅伴侣在内质网蛋白质质量控制中的作用缺陷。
2. **文献名称**：*DNAJB11 is a novel endoplasmic reticulum-associated protein involved in unfolded protein response*
**作者**：Yao Y, et al.
**摘要**：研究通过免疫共沉淀和Western blot(使用DNAJB11特异性抗体)证实DNAJB11与内质网应激相关蛋白(如BiP)相互作用，参与未折叠蛋白反应(UPR)，调控细胞应激适应机制。
3. **文献名称**：*Development of a monoclonal antibody against DNAJB11 for detection of renal tubule injury*
**作者**：Zhang L, et al.
**摘要**：报道了一种新型DNAJB11单克隆抗体的开发，通过免疫组化和ELISA验证其在肾小管损伤模型中的高表达，提示DNAJB11可作为急性肾损伤的生物标志物。
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注：以上文献为示例，实际引用时需根据具体研究补充完整信息(期刊、卷号、页码等)。如需更多文献，可进一步检索PubMed或NCBI数据库。
DNAJ homolog subfamily B member 11 (DNAJB11) is a member of the heat shock protein 40 (HSP40/DNAJ) family, which functions as a co-chaperone for HSP70 proteins. It plays a critical role in protein quality control by assisting in the folding, stabilization, and degradation of client proteins, particularly within the endoplasmic reticulum (ER). DNAJB11 is primarily localized to the ER lumen and is involved in the ER-associated degradation (ERAD) pathway, ensuring proper clearance of misfolded proteins to maintain cellular homeostasis.
Structurally, DNAJB11 contains a conserved J-domain that mediates interaction with HSP70. along with a glycine/phenylalanine-rich (G/F) domain and a C-terminal substrate-binding region. Its activity is crucial for cellular stress responses and has been implicated in diseases linked to protein misfolding, including certain cancers, neurodegenerative disorders, and polycystic kidney diseases. Notably, mutations in the DNAJB11 gene are associated with autosomal dominant tubulointerstitial kidney disease (ADTKD), highlighting its role in renal function.
Antibodies targeting DNAJB11 are widely used in research to study its expression, localization, and interaction networks. They are essential tools for Western blotting, immunohistochemistry, and immunofluorescence to explore DNAJB11's involvement in ER stress, proteostasis, and disease mechanisms. Commercially available DNAJB11 antibodies are typically validated for specificity across human and model organism tissues, aiding in both basic research and clinical investigations of ER-related pathologies.
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