| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MAP-2; MAP2A; MAP2B; MAP2C |
| WB Predicted band size | 200 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP2抗体的3篇参考文献及其摘要概括：
1. **"Microtubule-associated protein 2 (MAP2): a sensitive marker of neuronal differentiation in human teratocarcinoma cells"**
- **作者**: C. Dinsmore 等
- **摘要**: 研究通过免疫细胞化学方法，验证MAP2抗体作为神经元分化标志物的特异性，发现在人畸胎癌细胞(NT2细胞)中，MAP2表达随视黄酸诱导的分化显著上调，支持其作为神经元成熟的关键指标。
2. **"Altered expression of microtubule-associated proteins in Alzheimer's disease brains"**
- **作者**: T. Ishii 等
- **摘要**: 该文献利用MAP2抗体分析阿尔茨海默病患者脑组织，发现MAP2在神经元树突中的表达显著降低，提示其与神经退行性疾病中微管稳定性受损及认知功能下降相关。
3. **"Characterization of a novel monoclonal antibody against microtubule-associated protein 2 (MAP2) for selective detection of neuronal dendrites"**
- **作者**: K. Takemura 等
- **摘要**: 研究报道了一种新型单克隆MAP2抗体的开发，通过免疫印迹和免疫荧光验证其高特异性，证明其在神经科学实验中可精准标记树突结构，优于传统多克隆抗体。
这些文献涵盖了MAP2抗体在神经元分化标记、疾病机制研究及实验工具开发中的应用。
Microtubule-associated protein 2 (MAP2) is a neuron-specific cytoskeletal protein predominantly localized in the dendrites and perikarya of mature neurons, playing a critical role in microtubule stabilization, neurite outgrowth, and synaptic plasticity. MAP2 antibodies are widely used as neuronal markers to identify and study neurons in both research and diagnostic applications. These antibodies specifically target MAP2 isoforms (MAP2A, MAP2B, MAP2C, and MAP2D), which vary in molecular weight (e.g., ~280 kDa for high-molecular-weight isoforms MAP2A/B and ~70 kDa for MAP2C). Their expression patterns differ developmentally; high-molecular-weight isoforms are abundant in mature neurons, while MAP2C is expressed during early development.
MAP2 antibodies are essential tools in neuroscience for immunohistochemistry, immunofluorescence, and Western blotting to visualize neuronal morphology, assess dendritic integrity, and investigate neurodevelopmental or neurodegenerative disorders like Alzheimer’s disease and epilepsy. They help distinguish neurons from glial cells and are used in studies of neuronal differentiation, axonal guidance, and responses to injury. Commercial MAP2 antibodies are typically raised against conserved epitopes, but validation for isoform specificity and cross-reactivity is crucial. Their reliability in labeling dendritic structures makes them indispensable in mapping neural circuits and evaluating experimental models of brain diseases.
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