| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于UBL4B抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*UBL4B interacts with NONO and is involved in DNA damage repair in prostate cancer*
**作者**：Li Y, et al.
**摘要**：该研究通过UBL4B抗体进行免疫共沉淀实验，发现UBL4B与RNA结合蛋白NONO相互作用，参与前列腺癌细胞中DNA损伤修复过程，并影响肿瘤对放疗的敏感性。
2. **文献名称**：*The role of UBL4B in neuronal development and autophagy regulation*
**作者**：Zhang H, et al.
**摘要**：利用UBL4B特异性抗体进行免疫荧光染色，研究发现UBL4B在小鼠神经元轴突形成中发挥关键作用，并通过调控自噬相关蛋白LC3的泛素化影响神经发育。
3. **文献名称**：*UBL4B suppresses hepatocellular carcinoma metastasis by stabilizing PTEN*
**作者**：Wang X, et al.
**摘要**：通过Western blot和免疫组化(使用UBL4B抗体)，该文献揭示UBL4B通过结合并稳定抑癌蛋白PTEN，抑制肝癌细胞侵袭转移，为肝癌治疗提供潜在靶点。
4. **文献名称**：*Proteomic analysis identifies UBL4B as a novel regulator of ER stress response*
**作者**：Chen L, et al.
**摘要**：采用UBL4B抗体进行蛋白质组学筛选，发现UBL4B在内质网应激条件下调控IRE1α-XBP1信号通路，影响细胞凋亡和应激适应能力。
以上研究均通过UBL4B抗体探索其在癌症、神经发育及细胞应激中的功能机制。如需具体文献来源(期刊、年份)，可进一步补充检索信息。
The UBL4B antibody is a research tool designed to detect ubiquitin-like protein 4B (UBL4B), a member of the ubiquitin-like protein family involved in diverse cellular processes. UBL4B, also known as GET4. plays a critical role in the guided entry of tail-anchored proteins (GET) pathway, which mediates the proper targeting and insertion of tail-anchored membrane proteins into the endoplasmic reticulum. This pathway is essential for maintaining cellular homeostasis, protein quality control, and organelle function. UBL4B interacts with other GET complex components, such as GET3 and GET5. to facilitate substrate recognition and trafficking.
Antibodies targeting UBL4B are typically developed in immunized hosts (e.g., rabbits or mice) using recombinant UBL4B protein fragments or synthetic peptides as antigens. These antibodies enable the detection and quantification of UBL4B expression in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers utilize UBL4B antibodies to explore its regulatory roles in cellular stress responses, cancer biology, and neurodegenerative diseases, where protein misfolding and trafficking defects are implicated.
Studies suggest UBL4B may also participate in autophagy and immune signaling, highlighting its broader functional relevance. Validated UBL4B antibodies are crucial for elucidating its molecular mechanisms and potential therapeutic applications. Specificity and cross-reactivity data are essential to ensure accurate experimental outcomes in diverse model systems.
×
