| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HR6B; UBC2; HHR6B; RAD6B; E2-17kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于UBE2B抗体的3篇参考文献的模拟示例(基于领域内常见研究方向，具体文献需根据实际数据库检索确认)：
---
1. **文献名称**: *UBE2B is critical for DNA repair and spermatogenesis in mice*
**作者**: Roest HP, et al.
**摘要**: 本研究利用UBE2B抗体通过免疫印迹和免疫荧光技术，揭示了UBE2B基因敲除小鼠中精子发生缺陷及DNA损伤修复能力下降的机制，表明UBE2B在生殖细胞基因组稳定性中起关键作用。
---
2. **文献名称**: *Proteomic analysis of UBE2B interactions in Fanconi anemia pathway*
**作者**: van Twest S, et al.
**摘要**: 通过UBE2B抗体进行免疫共沉淀(Co-IP)实验，研究发现UBE2B与范可尼贫血核心复合物(FANCC/FANCG)存在直接互作，提示其在DNA交联损伤修复中的协同功能。
---
3. **文献名称**: *Dysregulation of UBE2B in colorectal cancer correlates with chemotherapy resistance*
**作者**: Li J, et al.
**摘要**: 该研究利用UBE2B抗体检测结直肠癌组织中UBE2B蛋白表达水平，发现其高表达与奥沙利铂耐药性显著相关，机制可能涉及泛素化依赖的凋亡通路抑制。
---
**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索确认。建议使用关键词“UBE2B antibody”、“UBE2B function”或结合具体研究领域(如“DNA repair”、“cancer”)进行精准查询。
The UBE2B antibody targets the ubiquitin-conjugating enzyme E2 B (UBE2B), a key component of the ubiquitin-proteasome system (UPS) responsible for protein degradation. UBE2B, also known as RAD6B, functions as an E2 enzyme that transfers ubiquitin to substrate proteins in conjunction with E3 ligases, marking them for proteasomal destruction. This post-translational modification regulates critical cellular processes, including DNA damage repair, cell cycle progression, and transcriptional regulation. UBE2B interacts with E3 ligases like RAD18 and BRE1 to mediate error-prone DNA repair pathways and histone H2B ubiquitination, respectively.
Antibodies against UBE2B are widely used in research to study its expression, localization, and molecular interactions. They enable techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation to explore UBE2B’s role in maintaining genomic stability and epigenetic regulation. Dysregulation of UBE2B has been implicated in cancer progression, male infertility, and neurodegenerative diseases, making it a biomarker of interest. Commercial UBE2B antibodies are typically validated for specificity across human and model organism samples. Recent studies also investigate UBE2B's potential as a therapeutic target, particularly in tumors with defective DNA repair mechanisms. Its conserved structure across eukaryotes underscores its fundamental role in cellular homeostasis.
×
