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产品描述：人Aldo-keto还原酶家族1成员B10(AKR1B10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AKR1B10含量。AKR1B10，全称醛酮还原酶家族1成员B10，是一种NAD(P)H依赖的氧化还原酶，参与脂肪代谢和羰基还原。其在肝癌细胞中表达显著高于正常肝细胞，可能与肝癌的发生发展有关。研究显示，AKR1B10基因沉默可抑制肝癌细胞增殖和凋亡，提示其可能成为肝癌治疗的潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于细胞生物学研究、肿瘤标志物表达分析、代谢性疾病机制探索等科研领域，为探究AKR1B10在病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AK1BA_HUMAN ELISA Kit; AKR1B10 ELISA Kit; AKR1B11 ELISA Kit; AKR1B12 ELISA Kit; Aldo keto reductase family 1 member B10 ELISA Kit; aldo keto reductase family 1 member B11 ELISA Kit; Aldo-keto reductase family 1 member B10 (aldose reductase) ELISA Kit; Aldo-keto reductase family 1 member B10 ELISA Kit; Aldo-keto reductase family 1 member B11 (aldose reductase-like) ELISA Kit; aldose reductase like 1 ELISA Kit; Aldose reductase like ELISA Kit; aldose reductase like peptide ELISA Kit; Aldose reductase related protein ELISA Kit; Aldose reductase-like ELISA Kit; Aldose reductase-related protein ELISA Kit; ALDRLn ELISA Kit; ARL 1 ELISA Kit; ARL-1 ELISA Kit; ARL1 ELISA Kit; ARL1 Human small intestine reductase ELISA Kit; ARP ELISA Kit; hARP ELISA Kit; HIS ELISA Kit; HSI ELISA Kit; SI reductase ELISA Kit; Small intestine reductase ELISA Kit
缩写：AKR1B10
Uniprot No.：O60218
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AKR1B10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-92 | |||||
回收率：
| The recovery of human AKR1B10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 85 | 81-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.1432.2532.1982.027201.5441.5341.5391.368101.0771.1641.1210.95050.7740.7920.7830.6122.50.5130.5230.5180.3471.250.3680.3980.3830.2120.6250.2730.2810.2770.10600.1700.1720.171 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AKR1B10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AKR1B10 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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