| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | hVPS16 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human VPS16 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于VPS16抗体的3篇示例参考文献(内容为虚构，仅作格式示例)：
---
1. **文献名称**: *VPS16 is required for ESCRT-III complex assembly and lysosomal protein sorting*
**作者**: Johnson A, et al.
**摘要**: 研究利用VPS16特异性抗体进行免疫沉淀和Western blot，发现VPS16与ESCRT-III亚基相互作用，调控溶酶体蛋白分选过程。抗体验证了VPS16在HEK293细胞中的表达及其复合体形成依赖性。
2. **文献名称**: *VPS16 antibody reveals its colocalization with autophagy markers in Parkinson's disease models*
**作者**: Chen L, et al.
**摘要**: 通过免疫荧光和VPS16抗体，作者发现VPS16与LC3共定位，提示其在自噬小体形成中的作用。研究在帕金森病神经元模型中观察到VPS16表达异常。
3. **文献名称**: *Structural insights into VPS16 function using cryo-EM and antibody-based validation*
**作者**: Müller S, et al.
**摘要**: 结合冷冻电镜和VPS16抗体的表位定位，揭示了VPS16在ESCRT复合体中的构象变化，抗体被用于验证突变体对膜重塑功能的影响。
---
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“VPS16 antibody”、“VPS16 ESCRT”或结合具体研究领域(如溶酶体、自噬)。实际研究中，抗体相关描述多出现于方法学部分(如抗体来源、克隆号、应用场景)。
VPS16 antibody targets the Vacuolar Protein Sorting 16 (VPS16) protein, a critical component of the Homotypic Fusion and Protein Sorting (HOPS) complex. This conserved multiprotein complex mediates membrane tethering and fusion in endolysosomal trafficking pathways. VPS16. along with other HOPS subunits (VPS11. VPS18. VPS33. VPS39. VPS41), plays a vital role in lysosome biogenesis, autophagosome-lysosome fusion, and endocytic cargo transport. Dysregulation of VPS16 is linked to lysosomal storage disorders, neurodegenerative diseases (e.g., Parkinson’s), and impaired cellular clearance mechanisms.
Researchers use VPS16 antibodies primarily to study intracellular membrane dynamics, lysosomal function, and autophagy in models ranging from yeast to mammalian cells. These antibodies enable detection of VPS16 expression levels, localization via immunofluorescence, and interaction analyses (e.g., co-immunoprecipitation). Commercial VPS16 antibodies are typically raised in rabbits or mice against specific epitopes, with validation in Western blotting, immunohistochemistry, or knockout validation assays. Recent studies also explore VPS16’s role in pathological contexts, such as protein aggregation diseases or viral infections exploiting endosomal pathways. Its involvement in maintaining lysosomal pH and enzyme activity further underscores its relevance in cell homeostasis research.
×
