首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管紧张素1-7(Ang1-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Ang1-7含量。Ang1-7是一种血管紧张素，作为肾素-血管紧张素系统（RAS）的组成部分，其作用与AngⅡ相反。研究显示，Ang1-7可通过调节Rac1、Rad等蛋白表达，抑制成纤维细胞合成细胞外基质，从而对心血管系统产生保护作用。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研中评估心血管疾病模型、代谢紊乱或器官损伤等实验条件下Ang1 - 7的动态变化，在高血压、糖尿病肾病等疾病机制研究中具有重要价值，满足实验室对生物活性分子定量分析的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Ang1-7
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 80-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-104 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-114 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 80-110 | |||||
回收率：
| The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 83-112 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12003.1213.1293.1252.9866002.5322.5222.5272.3883001.7391.7651.7521.6131501.0501.0411.0460.907750.6230.6420.6330.49437.50.4350.4470.4410.30218.750.2650.2540.2600.12100.1350.1430.139 | ||||||
货期：3-5 working days
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