| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DHHC-20; 4933421L13Rik |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZDHHC20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为假设性示例文献(非真实存在，仅供格式参考)：
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1. **文献名称**：ZDHHC20 Antibody Validation in Colorectal Cancer Tissues
**作者**：Li X, et al.
**摘要**：本研究通过Western blot和免疫组化验证了ZDHHC20抗体的特异性，发现其在结直肠癌组织中高表达，并与患者预后不良相关。
2. **文献名称**：Role of ZDHHC20 in Neuronal Palmitoylation: Antibody-Based Analysis
**作者**：Smith J, et al.
**摘要**：利用特异性ZDHHC20抗体，揭示了该酶在小鼠海马神经元中的棕榈酰化功能缺失会导致突触蛋白定位异常，影响神经信号传导。
3. **文献名称**：Development of a Novel ZDHHC20 Knockout Model Using CRISPR/Cas9 and Antibody Screening
**作者**：Wang Y, et al.
**摘要**：通过CRISPR/Cas9构建ZDHHC20敲除细胞系，结合多克隆抗体验证蛋白缺失，证实ZDHHC20在细胞膜受体运输中的关键作用。
4. **文献名称**：ZDHHC20 Antibody Application in Viral Infection Studies
**作者**：Garcia R, et al.
**摘要**：使用ZDHHC20抗体发现该蛋白与流感病毒包膜蛋白的棕榈酰化修饰相关，抑制其活性可降低病毒复制效率。
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注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索关键词“ZDHHC20 antibody”获取。
The ZDHHC20 antibody is a research tool designed to detect and study the ZDHHC20 protein, a member of the DHHC (Asp-His-His-Cys) family of palmitoyltransferases. These enzymes catalyze protein S-palmitoylation, a post-translational modification critical for regulating protein localization, stability, and interactions. ZDHHC20 contains conserved DHHC catalytic domains and transmembrane regions, enabling its role in lipid modification of target proteins. Research suggests its involvement in diverse cellular processes, including membrane trafficking, signal transduction, and immune responses. Dysregulation of ZDHHC20 has been implicated in diseases such as cancer, where altered palmitoylation may influence tumor progression and metastasis, and neurological disorders linked to synaptic protein dysfunction.
The antibody is typically generated using immunogens derived from specific ZDHHC20 epitopes (e.g., N-terminal or catalytic domains) and validated via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity is often confirmed using knockout cell lines or tissues. Commercial ZDHHC20 antibodies are widely used to explore its expression patterns, substrate interactions, and pathological roles. Recent studies also highlight its potential as a therapeutic target, driving demand for reliable detection tools. However, variability in antibody performance across experimental conditions underscores the need for rigorous validation. Overall, the ZDHHC20 antibody serves as a vital resource for elucidating the biological and clinical significance of protein palmitoylation.
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