Rabbit Polyclonal ATF6B Antibody

The ATF6B antibody is a crucial tool for studying the Activating Transcription Factor 6 Beta (ATF6β), a key protein involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. ATF6β, a member of the CREB/ATF family, shares structural homology with ATF6α but exhibits distinct regulatory roles. While ATF6α primarily mediates ER stress signaling by activating UPR target genes, ATF6β is thought to modulate this pathway through competitive interactions or tissue-specific functions, particularly in immune regulation and secretory cell types. The antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ATF6β expression, localization, and activation states. Researchers employ it to investigate diseases linked to ER stress dysregulation, such as neurodegenerative disorders, diabetes, and cancer. ATF6β antibodies often target epitopes in its N-terminal transcription-activating domain or C-terminal regulatory region. Validation typically includes knockout cell lines or tissues to confirm specificity, as cross-reactivity with ATF6α must be ruled out. Its applications extend to studying post-translational modifications, proteolytic processing during ER stress, and interactions with UPR components like BiP/GRP78. Commercial antibodies vary in host species (rabbit, mouse) and clonality, with recombinant monoclonal versions gaining traction for improved reproducibility.