| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | B12; CI-B12 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFB3抗体的3篇参考文献的简要信息(内容基于学术文献结构合理虚构，供参考)：
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1. **文献名称**: *NDUFB3 as a biomarker for mitochondrial dysfunction in Parkinson's disease*
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫组化技术，利用NDUFB3抗体分析帕金森患者脑组织中线粒体复合体I的蛋白表达水平。结果显示，NDUFB3表达显著降低，提示其可能作为线粒体功能异常的分子标志物。
2. **文献名称**: *Role of NDUFB3 in the assembly of mitochondrial complex I*
**作者**: Chen L, et al.
**摘要**: 作者通过siRNA敲低NDUFB3并结合NDUFB3抗体的免疫印迹分析，证明该蛋白对复合体I的组装至关重要。研究还利用免疫荧光定位NDUFB3在线粒体内膜中的分布。
3. **文献名称**: *NDUFB3 expression correlates with prognosis in hepatocellular carcinoma*
**作者**: Wang Y, et al.
**摘要**: 该研究使用NDUFB3抗体对肝癌组织进行免疫组化染色，发现NDUFB3低表达与患者生存率下降相关，提示其可能作为肝癌预后的潜在生物标志物。
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以上文献示例聚焦于NDUFB3抗体在疾病机制、蛋白功能及临床诊断中的应用。如需真实文献，建议通过PubMed或Google Scholar以“NDUFB3 antibody”为关键词进一步检索。
The NDUFB3 antibody is a tool used to detect the NDUFB3 protein, a subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. Encoded by the nuclear gene *NDUFB3*, this protein localizes to the inner mitochondrial membrane and plays a structural and functional role in Complex I assembly and activity. Complex I facilitates NADH oxidation, transferring electrons to ubiquinone while pumping protons to drive ATP synthesis. Dysregulation of Complex I subunits, including NDUFB3. is linked to mitochondrial disorders, neurodegenerative diseases, and cancer.
NDUFB3 antibodies are widely used in research to study protein expression, localization, and Complex I integrity via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help identify mutations or deficiencies in mitochondrial disorders such as Leigh syndrome or metabolic myopathies. Additionally, studies explore NDUFB3's role in cancer metabolism, as tumor cells often exhibit altered oxidative phosphorylation. These antibodies also aid in investigating cellular responses to oxidative stress, aging, and drug targeting of mitochondrial pathways. Validated for specificity, NDUFB3 antibodies are critical in molecular and cellular biology research focused on energy metabolism and disease mechanisms.
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