| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RPB12; RPABC4; RPB7.0; hRPB7.0; hsRPB10a; RPB10alpha; ABC10-alpha |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于POLR2K抗体的参考文献，按文献名称、作者和摘要概括整理：
1. **"Structural and functional analysis of the human RNA polymerase II subunit RPB7 (POLR2K)"**
- **作者**: Todone et al.
- **摘要**: 通过X射线晶体学和抗体结合实验，揭示POLR2K在RNA聚合酶II复合体中的结构定位及其在转录起始中的调控作用。
2. **"POLR2K interacts with HIF-1α to promote tumor angiogenesis in breast cancer"**
- **作者**: Wang et al.
- **摘要**: 利用POLR2K抗体进行免疫共沉淀和ChIP-seq，证明POLR2K与缺氧诱导因子HIF-1α结合，调控促血管生成基因表达，促进乳腺癌血管新生。
3. **"A CRISPR screen identifies POLR2K as a critical factor for RNA polymerase II stability"**
- **作者**: Martinez-Ruiz et al.
- **摘要**: 通过CRISPR筛选结合POLR2K抗体的Western blot验证，发现POLR2K缺失导致RNA聚合酶II复合体解体，影响全局转录活性。
4. **"Development and validation of a high-specificity POLR2K monoclonal antibody for diagnostic applications"**
- **作者**: Chen et al.
- **摘要**: 报道一种新型POLR2K单克隆抗体的开发，通过ELISA、免疫组化验证其特异性，并应用于肝癌组织样本中POLR2K异常表达的检测。
(注：以上文献信息为模拟示例，实际引用时请核对真实文献来源及内容准确性。)
The POLR2K antibody targets a subunit of RNA polymerase II (Pol II), the essential enzyme responsible for transcribing protein-coding genes in eukaryotes. POLR2K, also known as RPB10. is a highly conserved, small subunit (∼8.2 kDa) that forms part of the core structure of Pol II. Unlike larger catalytic subunits, POLR2K plays a structural role in stabilizing the polymerase complex and facilitating interactions with transcription regulatory factors. It is encoded by the POLR2K gene located on human chromosome 8q21.11.
Research using POLR2K antibodies primarily focuses on understanding transcriptional regulation mechanisms. These antibodies are employed in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to detect POLR2K expression, localize it within the nucleus, and study its association with Pol II during transcription initiation/elongation. Dysregulation of POLR2K has been implicated in cellular stress responses and diseases linked to transcription errors, including certain cancers and neurodegenerative disorders. For example, altered POLR2K expression patterns have been observed in hepatocellular carcinoma and glioblastoma.
Commercial POLR2K antibodies are typically raised against synthetic peptides corresponding to conserved regions of the human protein. Validations include specificity testing using knockout cell lines and cross-reactivity assessments across species. Researchers should optimize experimental conditions due to the subunit’s low molecular weight and potential cross-reactivity with other polymerase subunits. These reagents serve as critical tools for investigating fundamental gene expression processes and their pathological disruptions.
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