首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管生成素相关蛋白6(ANGPTL6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPTL6含量。ANGPTL6，也称为Angiopoietin-like 6，是一种与血管生成和细胞分化相关的蛋白质编码基因。其研究显示，ANGPTL6基因突变与家族性颅内动脉瘤形成有关，且在冠心病患者中，ANGPTL6水平与冠状动脉病变严重程度呈负相关，提示其可能作为潜在的治疗靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中探索ANGPTL6在代谢性疾病模型、血管生成机制或相关信号通路中的表达变化，为体外实验提供高效定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angptl6 ELISA kit; AgfAngiopoietin-related protein 6 ELISA kit; Angiopoietin-like protein 6 ELISA kit; Angiopoietin-related growth factor ELISA kit
缩写：ANGPTL6
Uniprot No.：Q8R0Z6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 82-96 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-107 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of mouse ANGPTL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-95 | ||||
| EDTA plasma (n=4) | 99 | 96-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6162.7932.7052.54620001.9272.0361.9821.82310001.2871.3881.3381.1795000.7690.7290.7490.5902500.5770.6040.5910.4321250.3940.4010.3980.23962.50.2470.2670.2570.09800.1640.1540.159 | ||||||
货期：3-5 working days
×
