| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | QCR6; UQCR8 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UQCRH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UQCRH抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*UQCRH downregulation promotes angiogenesis via mitochondrial oxidative stress in melanoma*
**作者**：Wang Y, et al.
**摘要**：该研究利用UQCRH抗体分析黑色素瘤中线粒体复合体III亚基UQCRH的表达，发现其下调通过激活ROS信号通路促进肿瘤血管生成，揭示了UQCRH在肿瘤代谢中的新机制。
2. **文献名称**：*A novel role for UQCRH in regulating cellular senescence*
**作者**：Kim H, et al.
**摘要**：通过免疫印迹(Western blot)和免疫荧光技术(使用UQCRH抗体)，作者证明UQCRH缺失导致线粒体功能异常，从而加速细胞衰老，为衰老相关疾病提供了潜在靶点。
3. **文献名称**：*UQCRH mutations cause mitochondrial complex III deficiency with neurological impairment*
**作者**：Ghezzi D, et al.
**摘要**：研究利用UQCRH抗体对患者细胞进行蛋白表达分析，发现UQCRH基因突变导致复合体III功能缺陷，引发线粒体脑肌病，强调了其在神经系统中的关键作用。
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注：上述文献为示例，实际文献需通过数据库(如PubMed)检索确认。建议结合具体研究需求补充近年的高相关论文。
The UQCRH (Ubiquinol-cytochrome c reductase hinge protein) antibody is a tool used to detect and study the UQCRH protein, a critical subunit of mitochondrial Complex III (cytochrome bc1 complex) in the electron transport chain (ETC). Located in the inner mitochondrial membrane, Complex III facilitates electron transfer from ubiquinol to cytochrome c while contributing to the proton gradient essential for ATP synthesis. UQCRH, encoded by the nuclear UQCRH gene, acts as a structural and functional stabilizer of Complex III, ensuring its proper assembly and interaction with other ETC components.
Research involving UQCRH antibodies focuses on understanding mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. For example, altered UQCRH expression has been observed in certain cancers, suggesting its role in tumor metabolism and apoptosis regulation. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression levels, localization, and interactions in cellular and tissue samples.
UQCRH antibodies are typically raised in host species (e.g., rabbits or mice) using immunogenic peptide sequences specific to conserved regions of the protein. Validation includes testing for specificity via knockdown/knockout controls. Their application extends to both basic research and clinical studies, aiding in the exploration of mitochondrial biology and therapeutic targeting of ETC-related pathologies.
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