首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管生成素相关蛋白7(ANGPTL7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPTL7含量。ANGPTL7是一个靶点。其背景上，它可能与某些生理或病理过程相关。在研究机制方面，目前可能着重探索其在疾病发生发展中的作用，比如对血管生成、细胞增殖等的影响，后续研究或为相关疾病治疗提供新的方向和策略。试剂盒检测范围为6.25 pg/mL-400 pg/mL，本试剂盒适用于小鼠模型中ANGPTL7蛋白水平的定量分析，可应用于心血管疾病、代谢调控或组织纤维化等领域的机制研究，例如在基因编辑模型或药物干预实验中评估ANGPTL7的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
缩写：ANGPTL7
Uniprot No.：Q8R1Q3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 95-109 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 93 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.7582.8902.8242.6322001.8231.9511.8871.6951001.0771.1381.1080.916500.6440.6210.6330.441250.4670.4510.4590.26712.50.3350.3360.3360.1446.250.2620.2550.2590.06700.1840.1990.192 | ||||||
货期：3-5 working days
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