| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TCTEX1D3 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于TCTE3相关研究的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: "TCTE3 is a conserved component of the dynein light chain family essential for sperm motility"
**作者**: Neesen, J. et al.
**摘要**: 该研究通过免疫沉淀和质谱分析，发现TCTE3作为动力蛋白轻链家族成员，在小鼠精子鞭毛中高表达。使用特异性抗体阻断TCTE3功能后，精子运动能力显著下降，表明其在轴丝动力蛋白组装中的关键作用。
2. **文献名称**: "Axonemal dynein intermediate chain light complex: structural role of TCTE3 in ciliary motility"
**作者**: King, S.M. & Patel-King, R.S.
**摘要**: 作者利用抗体验证TCTE3在纤毛动力蛋白复合体中的定位，发现其与DNAH5相互作用。抗体介导的功能抑制实验显示，TCTE3缺失导致纤毛摆动频率降低，提示其调控动力蛋白机械性能。
3. **文献名称**: "Mutation analysis of TCTE3 in male infertility patients with multiple morphological abnormalities of sperm flagella"
**作者**: Amiri-Yekta, A. et al.
**摘要**: 研究通过对不育患者基因测序，发现TCTE3突变与精子鞭毛畸形相关。Western blot和免疫荧光使用TCTE3抗体显示突变导致蛋白表达异常，证实其在人类精子发生中的必要性。
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**注意**：以上为模拟示例，实际文献需查阅PubMed、Web of Science等平台，结合关键词“TCTE3 antibody”或“TCTE3 dynein”获取。部分经典研究可能发表于《Journal of Cell Science》或《Human Molecular Genetics》等期刊。
The TCTE3 (T-complex-associated-testis-expressed 3) protein is a component of the outer dynein arm complex in cilia and flagella, playing a critical role in their motility. It belongs to the dynein family of motor proteins, which are essential for intracellular transport, ciliary beating, and sperm movement. Dysfunction of TCTE3 has been linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and male infertility due to defective sperm flagella.
Antibodies targeting TCTE3 are primarily used as research tools to study its expression, localization, and interaction within dynein complexes. They enable techniques like immunofluorescence, Western blotting, and immunoprecipitation, aiding in the characterization of ciliary ultrastructure and motility mechanisms. Commercially available TCTE3 antibodies are typically monoclonal or polyclonal, validated for specificity in model organisms (e.g., humans, mice).
Recent studies also explore TCTE3’s potential role beyond reproduction, including its involvement in cell signaling pathways and tumorigenesis. However, challenges remain in standardizing antibodies due to the protein’s structural complexity and low abundance in tissues. Ongoing research aims to clarify TCTE3’s molecular interactions and its relevance to therapeutic strategies for ciliopathies or fertility disorders.
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