| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SLC35E2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human SLC35E2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SLC35E2A抗体的3篇参考文献示例(注：由于实际研究可能较少，以下内容为模拟生成，供参考)：
1. **文献名称**：*SLC35E2A as a novel nucleotide sugar transporter involved in glycosylation pathways*
**作者**：Tanaka K, et al.
**摘要**：本研究鉴定了SLC35E2A作为新型核苷酸糖转运蛋白的功能，开发了特异性抗体用于其亚细胞定位分析，发现其在内质网-高尔基体运输中调控N-糖基化过程。
2. **文献名称**：*Antibody-based profiling of SLC35E2A expression in human cancers*
**作者**：Smith JL, et al.
**摘要**：通过制备多克隆抗体，分析了SLC35E2A在多种肿瘤组织中的表达差异，发现其与结直肠癌转移相关，提示其作为潜在生物标志物的价值。
3. **文献名称**：*Functional characterization of SLC35E2A knockout models using validated antibodies*
**作者**：Chen H, et al.
**摘要**：利用CRISPR技术构建SLC35E2A敲除细胞系，通过Western blot和免疫荧光验证抗体特异性，证明该蛋白缺失导致糖脂代谢异常及线粒体功能受损。
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**注意**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词"SLC35E2A antibody"或联系相关领域研究者获取。若研究较少，可扩展至SLC35家族其他成员或糖转运蛋白相关抗体文献。
The SLC35E2A antibody is a research tool designed to detect and study the solute carrier family 35 member E2A (SLC35E2A), a poorly characterized protein belonging to the SLC35 family of nucleotide sugar transporters. These transporters are critical for glycosylation processes, facilitating the translocation of nucleotide sugars (e.g., UDP-galactose, GDP-fucose) into the endoplasmic reticulum or Golgi apparatus for protein and lipid modification. SLC35E2A is predicted to contain multiple transmembrane domains and may play a role in cellular metabolism, though its specific substrates and biological functions remain unclear.
The antibody is typically developed in immunized hosts (e.g., rabbits or mice) using peptide antigens derived from SLC35E2A’s unique sequences. It enables the detection of endogenous SLC35E2A protein via techniques like Western blotting, immunofluorescence, or immunohistochemistry, aiding in studies of its expression patterns, subcellular localization, and potential involvement in diseases. Research suggests SLC35E2A may be linked to cancer progression or metabolic disorders, but evidence is limited.
Validation of antibody specificity often involves knockout cell lines or siRNA-mediated knockdown. Cross-reactivity with homologous proteins (e.g., SLC35E2B) must be ruled out. Current studies using this antibody are exploratory, focusing on elucidating SLC35E2A’s role in cellular physiology and pathology. Its application remains niche, reflecting the protein’s understudied status in the SLC transporter family.
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