首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠活化蛋白C(APC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APC含量。APC（腺瘤性息肉病基因）是一种肿瘤抑制基因，其突变与结直肠癌的发生密切相关。研究显示，APC的突变会导致Wnt/β-catenin信号通路异常激活，进而影响炎症信号参与结肠癌的发病过程。通过建立APC基因突变的小鼠肠癌模型，研究人员揭示了APC在结直肠癌发病机制中的关键作用，为开发新的治疗策略提供了理论基础。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，广泛应用于基础医学研究，如凝血/纤溶系统调控机制探索、炎症相关疾病动物模型评估、药物干预对APC通路的影响研究等，尤其适用于从多类型样本中系统性分析APC的动态变化，为心血管疾病、败血症等领域的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：APC
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6022.5882.5952.43320001.7761.8021.7891.62710001.0931.0781.0860.9245000.7150.6980.7070.5452500.4570.4340.4460.2841250.3040.3120.3080.14662.50.2440.2320.2380.07600.1640.1600.162 | ||||||
货期：3-5 working days
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