| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ACOT15 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与THEM5抗体相关的假设性参考文献及摘要概括(文献信息为虚构示例，仅供格式参考)：
1. **文献名称**：*"Characterization of THEM5-Specific Antibodies for Detection of Mitochondrial Thioesterase Activity"*
**作者**：Smith A. et al.
**摘要**：本研究开发并验证了针对THEM5蛋白的高特异性多克隆抗体，通过Western blot和免疫荧光证实其在哺乳动物细胞线粒体中的定位，并揭示THEM5在脂肪酸氧化中的关键作用。
2. **文献名称**：*"THEM5 Deficiency Alters Hepatic Lipid Metabolism: Insights from Antibody-Based Proteomic Analysis"*
**作者**：Chen L. et al.
**摘要**：利用THEM5抗体进行组织免疫组化分析，发现THEM5敲除小鼠肝脏中甘油三酯异常积累，表明该蛋白通过调控酰基-CoA水解影响脂代谢稳态。
3. **文献名称**：*"A Novel Monoclonal Antibody for THEM5 Reveals Its Tumor-Suppressive Role in Colorectal Cancer"*
**作者**：Wang X. et al.
**摘要**：报道一种高灵敏度抗THEM5单克隆抗体，应用于临床样本检测，证明THEM5低表达与结直肠癌进展相关，机制涉及ROS信号通路调控。
4. **文献名称**：*"Structural Insights into THEM5 via Epitope Mapping Using Domain-Specific Antibodies"*
**作者**：Kumar R. et al.
**摘要**：通过设计靶向THEM5不同结构域的抗体，结合质谱分析揭示其活性位点，为开发THEM5相关代谢疾病的靶向疗法提供依据。
(注：以上文献为模拟内容，实际研究中请通过PubMed或学术数据库检索真实文献。)
THEM5 (Thioesterase Superfamily Member 5) antibody is a research tool targeting the THEM5 protein, a member of the thioesterase superfamily involved in lipid metabolism. Thioesterases regulate cellular processes by hydrolyzing acyl-CoA thioesters, modulating fatty acid synthesis and degradation. THEM5. localized in mitochondria, plays a role in maintaining metabolic homeostasis by influencing acyl-CoA levels, which are critical for energy production and lipid signaling.
Studies suggest THEM5 is implicated in metabolic disorders, neurodegenerative diseases, and cancer, where altered lipid metabolism contributes to pathogenesis. For instance, THEM5 dysregulation has been linked to hepatic steatosis, insulin resistance, and tumor progression. The antibody enables detection of THEM5 expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into its physiological and pathological roles.
Recent research focuses on THEM5's potential as a biomarker or therapeutic target, particularly in contexts like obesity-related metabolic syndrome or chemotherapy resistance. However, its precise mechanisms remain under exploration. The development of THEM5 antibodies has thus become essential for advancing understanding of mitochondrial thioesterase functions and their relevance to human diseases.
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