| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | B17.2; DAP13 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NDUFA12抗体的虚构参考文献示例(基于学术文献常见结构模拟)：
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1. **文献名称**: *NDUFA12 Antibody Validation in Mitochondrial Complex I Assembly Studies*
**作者**: Thompson R, et al.
**摘要**: 本研究利用特异性NDUFA12抗体，通过免疫印迹和免疫共沉淀技术，验证了NDUFA12在线粒体复合物I组装中的关键作用。结果表明，NDUFA12缺失会导致复合物I稳定性下降，并与Leigh综合征相关突变相关。
2. **文献名称**: *NDUFA12 Expression Profiling in Neurodegenerative Disorders Using Monoclonal Antibodies*
**作者**: Chen L, et al.
**摘要**: 通过开发高特异性NDUFA12单克隆抗体，作者分析了阿尔茨海默病小鼠模型的脑组织样本，发现NDUFA12蛋白水平显著降低，提示其可能作为线粒体功能障碍的生物标志物。
3. **文献名称**: *A Novel NDUFA12 Antibody for Detecting Pathogenic Mutations in Cardiomyopathy*
**作者**: Gupta S, et al.
**摘要**: 本研究报道了一种新型NDUFA12抗体的临床应用，通过免疫组织化学和Western blot技术，在扩张型心肌病患者中检测到NDUFA12蛋白异常截短体，为遗传诊断提供了新工具。
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**注**: 以上内容为模拟生成，实际文献需通过PubMed/Google Scholar以关键词"NDUFA12 antibody"+"gene function"/"disease"检索。真实文献可参考线粒体疾病或复合物I相关研究(如涉及《Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease》等期刊)。
The NDUFA12 antibody is a research tool designed to detect and study the NDUFA12 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) within the electron transport chain. Complex I facilitates ATP production by transferring electrons from NADH to ubiquinone, coupled with proton pumping across the mitochondrial inner membrane. NDUFA12. encoded by the *NDUFA12* gene in humans, contributes to the structural stability and catalytic activity of Complex I. Dysfunction in Complex I subunits, including NDUFA12. is linked to mitochondrial disorders, neurodegenerative diseases, and cancer, making this protein a focus of metabolic and pathological research.
NDUFA12 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and abundance in tissues or cultured cells. These antibodies help investigate how NDUFA12 deficiencies or mutations impair mitochondrial respiration, ROS regulation, or cellular energy homeostasis. Studies involving NDUFA12 antibodies have provided insights into diseases such as Leigh syndrome, Leber hereditary optic neuropathy (LHON), and certain cancers, where altered Complex I activity is observed. Additionally, they aid in exploring therapeutic strategies targeting mitochondrial dysfunction. Validation of antibody specificity via knockout controls or siRNA knockdown is essential to ensure reliable results. Overall, NDUFA12 antibodies serve as vital tools in both basic and translational research focused on mitochondrial biology and associated diseases.
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